J. Lipid Res.
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A more recent version of this article appeared on July 1, 2005

Papers In Press, published online ahead of print May 1, 2005
J. Lipid Res., doi:10.1194/jlr.C500006-JLR200
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Submitted on February 17, 2005
Revised on April 19, 2005
Accepted on April 19, 2005

Misidentification of prostamides as prostaglandins

Michelle Glass, Jiwon Hong, Timothy A. Sato, and Murray D. Mitchell

Department of Pharmacology, University of Auckland, Auckland

Corresponding Author: m.glass{at}auckland.ac.nz

Prostaglandins and endogenous cannabinoid metabolites share the same lipid backbone with differing polar headgroups at exactly the position through which a large molecule is attached in order to provide antigenicity and thus raise antisera. Hence we hypothesized that antisera raised against prostaglandins linked to a large molecule such as BSA at the carboxyl functional group would also recognize endogenous cannabinoid metabolites and lead to highly misleading interpretations of data. We found major cross-reactivity of commercial antisera raised to prostaglandins with endocannabinoid metabolites. Furthermore in a well characterized cell line (WISH) or primary amnion tissue explants, endocannabinoid treatment leads to increased production of endocannabinoid metabolites as opposed to primary prostaglandins. This was apparent only after separation of products by thin layer chromatography since they measured as prostaglandins by radioimmunoassay. These findings have major implications for our interpretation of data in situations in which these prostaglandin like molecules are formed, and stress the need for chromatographic or spectrometric confirmation of prostaglandin production detected by antibody based methods.


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