J. Lipid Res.
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A more recent version of this article appeared on July 1, 2007

Papers In Press, published online ahead of print April 25, 2007
J. Lipid Res., doi:10.1194/jlr.C700002-JLR200
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Submitted on January 19, 2007
Revised on April 16, 2007
Accepted on April 24, 2007

Apolipoprotein A-V association with intracellular lipid droplets

Xiao Shu, Joanne Chan, Robert O Ryan, and Trudy M Forte

Children's Hospital Oakland Research Institute, Oakland, CA 94609

Corresponding Author: tforte{at}chori.org

Apolipoprotein (apo) A-V plays a key role in regulation of triglyceride (TG) metabolism. Given the very low concentration of apoA-V in plasma, we hypothesized that apoA-V may influence plasma TG levels by affecting the assembly and/or secretion of apoB-containing lipoproteins. When apoA-V was overexpressed in cultured Hep3B cells, neither the amount of apoB secreted nor the density distribution of apoB-containing lipoproteins was affected. Fluorescence microscopy and cell lysate immunoprecipitation studies revealed that apoA-V is not associated with apoB intracellularly, yet immunoprecipitation of apoA-V from the cell culture medium resulted in co-precipitation of apoB. These data suggest apoA-V association with apoB-containing lipoproteins is a post-secretory event. Confocal fluorescence microscopy revealed the presence of apoA-V in distinct cellular structures. Based on Nile Red staining we identified these structures to be intracellular lipid droplets. The data suggest that apoA-V has a unique association with cellular lipids and, therefore, may be involved in storage or mobilization of intracellular lipids.


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