J. Lipid Res.
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A more recent version of this article appeared on January 1, 2003

Papers In Press, published online ahead of print September 1, 2002
J. Lipid Res., doi:10.1194/jlr.D200013-JLR200
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Submitted on March 13, 2002
Revised on September 1, 2002
Accepted on August 20, 2002

Non-denaturing polyacrylamide gradient gel electrophoresis for the diagnosis of dysbetalipoproteinemia

Dirk J. Blom, Pamela Byrnes, Sheena Jones, and A. David Marais

Internal Medicine, University of Cape Town Health Sciences Faculty, Cape Town 7925

Corresponding Author: dmarais{at}uctgsh1.uct.ac.za

Dysbetalipoproteinemia, an uncommon but highly atherogenic mixed hyperlipidemia due to the accumulation of remnants of triglyceride-rich lipoproteins, is characterised by cholesterol-enriched VLDL that migrates in the b-position on agarose gels. The demonstration of a broad b-band on agarose gel electrophoresis of plasma is an insensitive method and ultracentrifugation is an impractical method of diagnosing this condition. Non-denaturing polyacrylamide gradient gel electrophoresis (PGGE) was investigated as a screening method for the diagnosis of dysbetalipoproteinemia. A minigel procedure separating the Sudan Black prestained apoB-containing lipoproteins on a 2-8 % polyacrylamide gel at 4 ° C overnight, was analysed for ultracentrifugally and genetically proven dysbetalipoproteinemic subjects as well as matched controls for mixed hyperlipidemia. Visual inspection revealed that the presence of only small VLDL- and IDL-like particles in untreated patients was highly sensitive (72 %) and specific (95%) for dysbetalipoproteinemia. Videodensitometric analysis of area under the curve for large and small VLDL, as well as IDL and LDL permitted even better discrimination in subjects whose profiles included some staining in the LDL-like region. A ratio of area under the curve of more than 0.5 for IDL/LDL allowed for a specificity of 100% and a sensitivity of 89% for the diagnosis of dysbetalipoproteinemia. This modified PGGE system may be useful in screening for dysbetalipoproteinemia.


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D. J. Blom, F. H. O'Neill, and A. D. Marais
Screening for Dysbetalipoproteinemia by Plasma Cholesterol and Apolipoprotein B Concentrations
Clin. Chem., May 1, 2005; 51(5): 904 - 907.
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