J. Lipid Res. Did you know there is a large type edition? Click here.
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

A more recent version of this article appeared on December 1, 2002

Papers In Press, published online ahead of print September 1, 2002
J. Lipid Res., doi:10.1194/jlr.D200020-JLR200
This Article
Right arrow Full Text (Accepted Manuscript)
Right arrow All Versions of this Article:
D200020-JLR200v1
43/12/2180    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Su, Y. R.
Right arrow Articles by Fazio, S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Su, Y. R.
Right arrow Articles by Fazio, S.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Submitted on May 21, 2002
Revised on August 21, 2002
Accepted on August 27, 2002

Rapid quantification of murine ABC mRNAs by real time reverse transcription-polymerase chain reaction

Yan Ru Su, MacRae F. Linton, and Sergio Fazio

Department of Medicine, Division of Cardiology, Vanderbilt University, Nashville, TN 37232-6300

Corresponding Author: Yan.Ru.Su{at}mcmail.vanderbilt.edu

Abstract Several ATP-binding cassette (ABC) transporters are critically involved in cholesterol and phospholipid efflux, reverse cholesterol transport, and play an important role in the development of atherosclerosis. Quantification of ABC mRNA is important in studying the regulation of cellular cholesterol homeostasis and mechanisms related to the pathogenesis of atherosclerosis. We have developed a one-step real time quantitative reverse transcription-Polymerase Chain Reaction (RT-PCR) method for measuring mRNA levels of ABCA1, ABCG1 and ABCA2 in murine tissues using the TaqManTM technology. It has significant methodological benefits when compared to classic Northern blotting or semi-quantitative RT-PCR analysis. Using this method, we found high expression levels of ABCA1 in liver and macrophages, and of ABCG1 in the brain and macrophages. The expression of ABCA1 and ABCG1 were further induced in macrophages loaded with acLDL. In contrast, ABCA2 was expressed exclusively in the brain with low expression levels in the macrophages. This method provides a rapid, highly sensitive, specific and reproducible quantification of ABC mRNA, and can be performed with nanograms of total RNA sample, thus making it a superior method for studying the regulation of ABC transporters in cholesterol efflux and its role in the pathogenesis of atherosclerosis in murine models.


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 Arterioscler. Thromb. Vasc. Bio.Home page
Y. R. Su, J. L. Blakemore, Y. Zhang, M. F. Linton, and S. Fazio
Lentiviral Transduction of ApoAI Into Hematopoietic Progenitor Cells and Macrophages: Applications to Cell Therapy of Atherosclerosis
Arterioscler. Thromb. Vasc. Biol., August 1, 2008; 28(8): 1439 - 1446.
[Abstract] [Full Text] [PDF]

Home page
 FASEB J.Home page
B. Gigante, G. Morlino, M. T. Gentile, M. G. Persico, and S. De Falco
Plgf-/-eNos-/- mice show defective angiogenesis associated with increased oxidative stress in response to tissue ischemia
FASEB J, May 1, 2006; 20(7): 970 - 972.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
H. Ando, H. Yanagihara, Y. Hayashi, Y. Obi, S. Tsuruoka, T. Takamura, S. Kaneko, and A. Fujimura
Rhythmic Messenger Ribonucleic Acid Expression of Clock Genes and Adipocytokines in Mouse Visceral Adipose Tissue
Endocrinology, December 1, 2005; 146(12): 5631 - 5636.
[Abstract] [Full Text] [PDF]

Home page
 J. Lipid Res.Home page
S. L. Sabol, H. B. Brewer Jr., and S. Santamarina-Fojo
The human ABCG1 gene: identification of LXR response elements that modulate expression in macrophages and liver
J. Lipid Res., October 1, 2005; 46(10): 2151 - 2167.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
B. Zhang, J. Groffen, and N. Heisterkamp
Resistance to farnesyltransferase inhibitors in Bcr/Abl-positive lymphoblastic leukemia by increased expression of a novel ABC transporter homolog ATP11a
Blood, August 15, 2005; 106(4): 1355 - 1361.
[Abstract] [Full Text] [PDF]

Home page
 Antimicrob. Agents Chemother.Home page
M. Viveiros, A. Jesus, M. Brito, C. Leandro, M. Martins, D. Ordway, A. M. Molnar, J. Molnar, and L. Amaral
Inducement and Reversal of Tetracycline Resistance in Escherichia coli K-12 and Expression of Proton Gradient-Dependent Multidrug Efflux Pump Genes
Antimicrob. Agents Chemother., August 1, 2005; 49(8): 3578 - 3582.
[Abstract] [Full Text] [PDF]

Home page
 Arterioscler. Thromb. Vasc. Bio.Home page
S. Ragozin, A. Niemeier, A. Laatsch, B. Loeffler, M. Merkel, U. Beisiegel, and J. Heeren
Knockdown of Hepatic ABCA1 by RNA Interference Decreases Plasma HDL Cholesterol Levels and Influences Postprandial Lipemia in Mice
Arterioscler. Thromb. Vasc. Biol., July 1, 2005; 25(7): 1433 - 1438.
[Abstract] [Full Text] [PDF]

Home page
 CirculationHome page
Y. R. Su, D. E. Dove, A. S. Major, A. H. Hasty, B. Boone, M. F. Linton, and S. Fazio
Reduced ABCA1-Mediated Cholesterol Efflux and Accelerated Atherosclerosis in Apolipoprotein E-Deficient Mice Lacking Macrophage-Derived ACAT1
Circulation, May 10, 2005; 111(18): 2373 - 2381.
[Abstract] [Full Text] [PDF]

Home page
 Arterioscler. Thromb. Vasc. Bio.Home page
D. E. Dove, Y. R. Su, W. Zhang, W. G. Jerome, L. L. Swift, M. F. Linton, and S. Fazio
ACAT1 Deficiency Disrupts Cholesterol Efflux and Alters Cellular Morphology in Macrophages
Arterioscler. Thromb. Vasc. Biol., January 1, 2005; 25(1): 128 - 134.
[Abstract] [Full Text] [PDF]

Home page
 J. Pharmacol. Exp. Ther.Home page
H. Ando, S. Tsuruoka, H. Yamamoto, T. Takamura, S. Kaneko, and A. Fujimura
Effects of Pravastatin on the Expression of ATP-Binding Cassette Transporter A1
J. Pharmacol. Exp. Ther., October 1, 2004; 311(1): 420 - 425.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
Y. Fu, A. Hoang, G. Escher, R. G. Parton, Z. Krozowski, and D. Sviridov
Expression of Caveolin-1 Enhances Cholesterol Efflux in Hepatic Cells
J. Biol. Chem., April 2, 2004; 279(14): 14140 - 14146.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
L. Gate, R. S. Majumdar, A. Lunk, and K. D. Tew
Increased Myeloproliferation in Glutathione S-Transferase {pi}-deficient Mice Is Associated with a Deregulation of JNK and Janus Kinase/STAT Pathways
J. Biol. Chem., March 5, 2004; 279(10): 8608 - 8616.
[Abstract] [Full Text] [PDF]

Home page
 CirculationHome page
W. Zhang, P. G. Yancey, Y. R. Su, V. R. Babaev, Y. Zhang, S. Fazio, and M. F. Linton
Inactivation of Macrophage Scavenger Receptor Class B Type I Promotes Atherosclerotic Lesion Development in Apolipoprotein E-Deficient Mice
Circulation, November 4, 2003; 108(18): 2258 - 2263.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
S. Weng, L. Zemany, K. N. Standley, D. V. Novack, M. La Regina, C. Bernal-Mizrachi, T. Coleman, and C. F. Semenkovich
{beta}3 integrin deficiency promotes atherosclerosis and pulmonary inflammation in high-fat-fed, hyperlipidemic mice
PNAS, May 27, 2003; 100(11): 6730 - 6735.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
 All ASBMB Journals   Journal of Biological Chemistry 
 Molecular and Cellular Proteomics   ASBMB Today 
Copyright © 2002 by the American Society for Biochemistry and Molecular Biology.