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Papers In Press, published online ahead of print January 16, 2003
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Center for Metabolism & Endocrinology, Dept of Medicine, Karolinska Institute, Stockholm, Sweden S-141 86
Corresponding Author: mats.rudling{at}cnt.ki.se
We describe an accurate method to monitor the enzymatic activity of hepatic cholesterol 7
Revised on January 15, 2003
Accepted on January 15, 2003
Monitoring hepatic cholesterol 7
-hydroxylase activity by assay of the stable bile acid intermediate 7-hydroxy-4-cholesten-3-one in peripheral blood
-hydroxylase (C7OH; CYP7A1), the rate-limiting and major regulatory enzyme in the synthesis of bile acids. Assay of 7-hydroxy-4-cholesten-3-one (C4), an intermediate in bile acid synthesis, revealed that the level of C4 in peripheral blood serum or plasma showed a strong correlation to the enzymatic activity of hepatic C7OH, both at steady state conditions (r = 0.929)as well as during the rapid changes that occur during the diurnal phases. This assay should be of value in order to clarify the regulation of bile acid synthesis in vivo in laboratory animals and humans, since it allows for the monitoring of hepatic C7OH activity using peripheral blood samples.
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