A novel and simple method for quantification of small dense low-density lipoprotein

Tsutomu Hirano, Yasuki Ito, Haruhisa Saegusa, and Gen Yoshino

First Department of Internal Medicine, Showa University School of Medicine, Tokyo 142-8666

Corresponding Author: hirano{at}med.showa-u.ac.jp

A preponderance of small dense (sd) low-density liporotein (LDL) is strongly associated with the development of coronary heart disease, but the method for the measurement of sd LDL are too laborious for clinical use. We report a simple method for the qua ntification of sd LDL, which is applicable to autoanalyser. This method consists of two steps; first, to precipitate the lipoprotein of density (d)<1.044 g/ml using heparin-magnesium; and second, to measure LDL-cholesterol in the supernatant by the homog enous method or apolipoprotein (apo) B by an immunoturbidometric assay. The cholesterol and apo B values obtaining the precipitation method (45±26 and 33±20 mg/dl, respectively) were similar to those obtained in the lipoprotein d=1.044-1.063 separated by ultracentrifugation (42±22 and 31±17), and there was an excellent correlation between the two methods for sd LDL-cholesterol (y=1.05X+1, r=0.88, n=69) and -apo B (y=1.07X, r=0.90). Sd LDL values had a significant inverse correlation with LDL size. A high correlation was found between sd LDL-cholesterol and -apo B values (r=0.94). Sd LDL value was related to triglyceride, apo B, and LDL-cholesterol, but not to the buoyant LDL level. These results suggest this precipitation method is a simple and rapid method for the measurement of sd LDL concentration.

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