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A more recent version of this article appeared on September 1, 2003

Papers In Press, published online ahead of print June 16, 2003
J. Lipid Res., doi:10.1194/jlr.D300009-JLR200
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Submitted on March 11, 2003
Revised on June 3, 2003
Accepted on June 10, 2003

Quantitative analysis of fatty acid precursors in marine samples: direct conversion of wax ester alcohols and dimethylacetals to fatty acid methyl esters

Suzanne M. Budge and Sara J. Iverson

Department of Biology, Dalhousie University, Halifax, Nova Scotia B3H 4J1

Corresponding Author: budges{at}dal.ca

To apply fatty acid analyses to the study of foraging ecology and diet determination, all compounds that may be deposited as fatty acids in a predator must be quantified in the prey. These compounds include the usual fatty acids in acyl lipids, but also the alcohols of wax esters and the vinyl ethers of plasmalogens. In routine fatty acid analysis, samples are extracted and transesterified (methylated), resulting in the formation of fatty acid methyl esters (FAME); however, fatty alcohols and dimethyl acetals (DMA) are also generated if wax esters or plasmalogens are present. Here, we present a new method using a modified Jones' reagent to oxidize these alcohols and DMA to free fatty acids (FFA). These FFA are then easily methylated and quantitatively recombined with FAME from the same sample. This generates a fatty acid signature of prey that is equivalent to that which the predator has available for deposition upon digestion of that prey. This method is validated with alcohol and DMA standards. Its application to typical marine samples is also presented, demonstrating the change in effective fatty acid signature after inclusion of fatty acids derived from wax esters and plasmalogen


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J. V. O'Fallon, J. R. Busboom, M. L. Nelson, and C. T. Gaskins
A direct method for fatty acid methyl ester synthesis: Application to wet meat tissues, oils, and feedstuffs
J Anim Sci, June 1, 2007; 85(6): 1511 - 1521.
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