J. Lipid Res.
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

A more recent version of this article appeared on February 1, 2004

Papers In Press, published online ahead of print October 16, 2003
J. Lipid Res., doi:10.1194/jlr.D300024-JLR200
This Article
Right arrow Full Text (Accepted Manuscript)
Right arrow All Versions of this Article:
D300024-JLR200v1
45/2/396    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Mizoguchi, T.
Right arrow Articles by Kosi, T.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Mizoguchi, T.
Right arrow Articles by Kosi, T.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Submitted on July 2, 2003
Revised on October 8, 2003
Accepted on October 14, 2003

A method of direct measurement for the enzymatic determination of cholesterol esters

Toshimi Mizoguchi, Toshiyuki Edano, and Tomoyuki Kosi

Tokyo Research Laboratories,, Pharmoceutical Division, Tokyo 189-0022

Corresponding Author: t-mizogt{at}kowa.co.jp

A direct measurement method for the enzymatic determination of cholesterol esters (CE) without measuring total cholesterol (TC) and free cholesterol (FC) are described. In the first step, hydrogen peroxide generated by cholesterol oxidase from FC was decomposed by catalase. In the second step, CE was measured by enzymatic determination using a colorimetric method or fluorometric method. The measurement sensitivity of fluorometric method was more than twenty times compared to colorimetric method. Optimal conditions of the assay were determined and examples of measured CE in human plasma, rat liver, and cultured cells were indicated. The method of directly measuring CE was simple and has exceptional reproducibility compared to the technique of subtracting FC from TC using each measured TC and F


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 J. Virol.Home page
M. Prior, S. Lehmann, M.-S. Sy, B. Molloy, and H. E. M. McMahon
Cyclodextrins Inhibit Replication of Scrapie Prion Protein in Cell Culture
J. Virol., October 15, 2007; 81(20): 11195 - 11207.
[Abstract] [Full Text] [PDF]

Home page
 J. Nutr.Home page
C. T. Damsgaard, L. Schack-Nielsen, K. F. Michaelsen, M.-B. Fruekilde, O. Hels, and L. Lauritzen
Fish Oil Affects Blood Pressure and the Plasma Lipid Profile in Healthy Danish Infants
J. Nutr., January 1, 2006; 136(1): 94 - 99.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
 All ASBMB Journals   Journal of Biological Chemistry 
 Molecular and Cellular Proteomics   ASBMB Today 
Copyright © 2003 by the American Society for Biochemistry and Molecular Biology.