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A more recent version of this article appeared on April 1, 2004

Papers In Press, published online ahead of print January 16, 2004
J. Lipid Res., doi:10.1194/jlr.D300035-JLR200
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Submitted on November 3, 2003
Revised on January 7, 2004
Accepted on January 13, 2004

Improved method for making nondenaturing composite gradient gels for the electrophoretic separation of lipoproteins

David L. Rainwater, Perry H. Moore . Jr, and Israel O. Gamboa

Department of Genetics, Southwest Foundation Biomedical Research, San Antonio, TX 78245-0549

Corresponding Author: david{at}darwin.sfbr.org

Nondenaturing gradient gel electrophoresis continues to be used widely for resolution and characterization of lipoprotein subclasses. Methods for making such gels in the laboratory have been published, but occasionally samples do not display uniform mobilities for all lanes in a gel made in the laboratory. To help overcome this limitation, we recommend a modification - addition of a sucrose gradient - that significantly improves within-gel variation in protein mobility.


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