Submitted on December 28, 2003
Revised on March 30, 2004
Accepted on April 6, 2004
Bacterial phospholipid molecular species analysis by ion-pair reversed-phase liquid chromatography/electrospray ionization/mass spectrometry
Nicolas Mazzella, Josiane Molinet, Agung Dhamar Syakti, Alain Dodi, Pierre Doumenq, Jacques Artaud, and Jean-Claude Bertrand
Laboratoire de Chimie Analytique de l'Environnement, Université d'Aix-Marseille III, Aix en Provence, Aix en Provence 13545
Corresponding Author: pierre.doumenq{at}univ.u-3mrs.fr
This work set out an optimization of the detection and separation of several phospholipid molecular species on a reversed phase column with the use of an ESI/MS compatible counter-ion. Afterward an application of this technique concerned a qualitative and quantitative analysis of bacterial membrane phospholipids extracted from Corynebacterium sp. strain 8. The phospholipid classes of strain 8 were identified as phosphatidylglycerol (PG), phosphatidylinositol (PI), cardiolipin (DPG) and a peculiar lipid compound: acyl phosphatidylglycerol (APG). Besides, most of the molecular species structures were elucidated and regarding PG, the fatty acid positions were clearly determined with the calculation of the sn-2/sn-1 intensity ratio of the fatty acyl chains fragments.
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