J. Lipid Res. Neurobiology of Lipids (ISSN1683-5506)
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

A more recent version of this article appeared on July 1, 2004

Papers In Press, published online ahead of print April 21, 2004
J. Lipid Res., doi:10.1194/jlr.D400001-JLR200
This Article
Right arrow Full Text (Accepted Manuscript)
Right arrow All Versions of this Article:
D400001-JLR200v1
45/7/1364    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Garbow, J. R.
Right arrow Articles by Schonfeld, G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Garbow, J. R.
Right arrow Articles by Schonfeld, G.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Submitted on January 20, 2004
Revised on April 14, 2004
Accepted on April 18, 2004

In vivo MRS measurement of liver lipid levels in mice

Joel R. Garbow, Xiaobo Lin, Nobuhiro Sakata, Zhouji Chen, Dukho Koh, and Gustav Schonfeld

Biomedical MR Laboratory, Washington University, St. Louis, MO 63110

Corresponding Author: garbow{at}wustl.edu

A magnetic resonance spectroscopy (MRS) procedure for in vivo measurement of lipid levels in mouse liver is described and validated. The method uses respiratory-gated, localized spectroscopy to collect proton spectra from voxels within the mouse liver. Bayesian Probability Theory analysis of these spectra allows the relative intensities of the lipid and water resonances within the liver to be accurately measured. All spectral data were corrected for measured spin-spin (T2) relaxation. A total of 48 mice were used in this study, including wild-type mice and two different transgenics. Different groups of these mice were fed high-fat or low-fat diets or liquid diets, with and without the addition of alcohol. Proton spectra were collected at baseline and, subsequently, every 4 weeks for up to 16 weeks. Immediately following the last MRS measurement, mice were sacrificed and their livers analyzed for triglyceride level by conventional wet-chemistry methods. The excellent correlation between in vivo MRS and ex vivo wet-chemistry determinations of liver lipid validates the MRS method. The results clearly demonstrate that in vivo MRS will be an extremely valuable technique for longitudinal studies aimed at providing important insights into the genetic, environmental, and dietary factors affecting fat deposition and accumulation within the mouse liver.


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 RadiologyHome page
R. J. H. Borra, S. Salo, K. Dean, R. Lautamaki, P. Nuutila, M. Komu, and R. Parkkola
Nonalcoholic Fatty Liver Disease: Rapid Evaluation of Liver Fat Content with In-Phase and Out-of-Phase MR Imaging
Radiology, November 18, 2008; (2008) 2501071934.
[Abstract] [Full Text]

Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
S. Rigazio, H.-R. Lehto, H. Tuunanen, K. Nagren, M. Kankaanpaa, C. Simi, R. Borra, A. G. Naum, R. Parkkola, J. Knuuti, et al.
The lowering of hepatic fatty acid uptake improves liver function and insulin sensitivity without affecting hepatic fat content in humans
Am J Physiol Endocrinol Metab, August 1, 2008; 295(2): E413 - E419.
[Abstract] [Full Text] [PDF]

Home page
 J. Lipid Res.Home page
K. Strobel, J. van den Hoff, and J. Pietzsch
Localized proton magnetic resonance spectroscopy of lipids in adipose tissue at high spatial resolution in mice in vivo
J. Lipid Res., February 1, 2008; 49(2): 473 - 480.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
 All ASBMB Journals   Journal of Biological Chemistry 
 Molecular and Cellular Proteomics   ASBMB Today 
Copyright © 2004 by the American Society for Biochemistry and Molecular Biology.