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Papers In Press, published online ahead of print May 16, 2005
J. Lipid Res., doi:10.1194/jlr.D500010-JLR200
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Department of Pharmacology, Vanderbilt University Medical School, Nashville, TN 37232-6600
Corresponding Author: alex.brown{at}vanderbilt.edu
The development of a new mass spectrometric lipid profiling methodology permits the identification of cellular PIP/PIP2/PIP3 species that includes the fatty acyl composition. Using ESI-MS we were able to resolve and identify 28 PIP and PIP2 as well as 8 PIP3 compounds from RAW 264.7 or primary murine macrophage cell extracts. Analysis of PIP profiles following agonist stimulation of cells reveals the generation of differential PIP3 species, and permits us to propose a novel means for regulation and specificity in signaling through PIP3. This is the first reported identification of intact, cellular PIP3 by mass spectral analysis. The ability to analyze the fatty acyl chain composition of signaling lipids initiates new venues for investigation of the processes in which specific polyphosphoinositide species mediate.
Revised on May 5, 2005
Accepted on May 7, 2005
A targeted mass spectrometric analysis of phosphatidylinositol phosphate species
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