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A more recent version of this article appeared on September 1, 2005

Papers In Press, published online ahead of print July 1, 2005
J. Lipid Res., doi:10.1194/jlr.D500019-JLR200
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Submitted on April 19, 2005
Revised on June 16, 2005
Accepted on June 23, 2005

Determining hepatic triglyceride production in mice: comparison of Poloxamer 407 with Triton WR-1339

John S. Millar, Debra A. Cromley, Mary G. McCoy, Daniel J. Rader, and Jeffrey T. Billheimer

Institute for Translational Medicine and Therapeutics, University of Pennsylvania, Philadelphia, PA 19104

Corresponding Author: billheij{at}mail.med.upenn.edu

Triglyceride (TG), a water insoluble energy-rich lipid, is secreted by the liver as part of very low density lipoproteins to supply energy to extrahepatic tissues. Overproduction of VLDL is associated with increased risk of cardiovascular heart disease, which has renewed interest in factors that affect hepatic TG production. The triglyceride production rate is determined by measuring temporal increases in plasma TG under conditions in which TG hydrolysis by lipoprotein lipase is inhibited. The non-ionic detergent, Triton WR-1339 (Triton), has commonly been used to inhibit lipoprotein lipase for this purpose. Triton, in addition to inhibition of TG hydrolysis, has properties that have the potential to adversely influence lipoprotein metabolism. Another non-ionic detergent, Poloxamer 407 (P-407), also inhibits lipoprotein lipase. In these studies we demonstrate that P-407 is comparable to Triton in the determination of TG production but without the unwanted side effects of Triton.


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