J. Lipid Res.
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

A more recent version of this article appeared on October 1, 2006

Papers In Press, published online ahead of print July 21, 2006
J. Lipid Res., doi:10.1194/jlr.D600018-JLR200
This Article
Right arrow Full Text (Accepted Manuscript)
Right arrow All Versions of this Article:
D600018-JLR200v1
47/10/2340    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Saini, G. S.
Right arrow Articles by Paliwal, J. K.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Saini, G. S.
Right arrow Articles by Paliwal, J. K.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Submitted on May 15, 2006
Revised on July 21, 2006
Accepted on July 21, 2006

Validation of LC-MS/MS method for quantification of mevalonic acid in human plasma and an approach to differentiate recovery and matrix effect

G. S. Saini, T. A. Wani, A. Gautam, B. Varshney, T. Ahmed, K. S. Rajan, K. K. Pillai, and J. K. Paliwal

Ranbaxy Research Laboratories, Gurgaon, Haryana 122015

Corresponding Author: gurpreet.saini{at}ranbaxy.com

A simple, specific and sufficiently sensitive LC-MS/MS (negative-ion ESI) methodology to determine mevalonic acid (MVA) in human plasma is described, and its application to analysis of rat plasma MVA levels following rosuvastatin administration is demonstrated. The method was validated over the linearity range of 0.5-50.0 ng/mL (r2 > 0.99) using deuterated MVA (D7-MVA) as internal standard (IS). The lower limit of quantification (LLOQ) was 0.5 ng/mL. The assay procedure involved isolation of MVA from plasma samples using solid phase extraction. Chromatographic separation was achieved on a HyPurity Advance column with a mobile phase consisted of ammonium formate buffer (10 mM, pH 8.0) and acetonitrile (70:30, v/v). Excellent precision and accuracy was observed. MVA and deuterated mevalonolactone (D7-MVAL) were stable in water and plasma under different storage and processing conditions. The recovery observed was low, which was attributable to significant matrix effect. A significant decrease (30-40%, p < 0.05) was observed in rat plasma MVA levels following rosuvastatin administration.


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 J. Lipid Res.Home page
S. M. Ludke, U. Flenker, W. Schanzer, and D. Schomburg
Stable carbon isotope discrimination by human 3-hydroxy-3-methylglutaryl-coenzyme A reductase
J. Lipid Res., December 1, 2008; 49(12): 2620 - 2626.
[Abstract] [Full Text] [PDF]

Home page
 J. Lipid Res.Home page
A. Honda, Y. Mizokami, Y. Matsuzaki, T. Ikegami, M. Doy, and H. Miyazaki
Highly sensitive assay of HMG-CoA reductase activity by LC-ESI-MS/MS
J. Lipid Res., May 1, 2007; 48(5): 1212 - 1220.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
 All ASBMB Journals   Journal of Biological Chemistry 
 Molecular and Cellular Proteomics   ASBMB Today 
Copyright © 2006 by the American Society for Biochemistry and Molecular Biology.