J. Lipid Res.
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A more recent version of this article appeared on January 1, 2007

Papers In Press, published online ahead of print October 19, 2006
J. Lipid Res., doi:10.1194/jlr.D600034-JLR200
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Submitted on August 31, 2006
Revised on October 10, 2006
Accepted on October 19, 2006

Rapid characterization of fatty acyl composition of complex lipids by collision-induced dissociation time-of-flight mass spectrometry

Steven Wynn Esch, Pamela Tamura, Alexis A. Sparks, Mary R. Roth, Shivakumar P. Devaiah, Ernst Heinz, Xuemin Wang, Todd D. Williams, and Ruth Welti

Division of Biology, Kansas State University, Manhattan, KS 66506-4901

Corresponding Author: welti{at}ksu.edu

Profiling of leaf extracts from mutants of Arabidopsis with defects in lipid desaturation demonstrates the utility of collision-induced dissociation-time of flight (CID-TOF) mass spectrometry (MS) for screening biological samples for fatty acid compositional alterations. CID-TOF MS utilizes the collision cell of a quadrupole-time of flight (Q-TOF) mass spectrometer to simultaneously fragment all the ions produced by an ionization source. Electrospray (ESI) CID-TOF MS in the negative mode can be used to analyze fatty acyl anions derived from complex lipids as well as free fatty acids. Although acyl anion yield is shown to be a function of the lipid class and position on the glycerol backbone, acyl compositional profiles can be determined, and the TOF detector provides resolution of nominally isobaric acyl species in the profiles. Good precision is obtained when data are acquired for approximately 1 minute per sample.


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[Abstract] [Full Text] [PDF]


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