J. Lipid Res. Neurobiology of Lipids (ISSN1683-5506)
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A more recent version of this article appeared on November 1, 2007

Papers In Press, published online ahead of print August 23, 2007
J. Lipid Res., doi:10.1194/jlr.D700017-JLR200
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Submitted on June 18, 2007
Revised on August 21, 2007
Accepted on August 23, 2007

A simple and rapid method to assay triacylglycerol in cells and tissues

Danielle M Schwartz and Nathan E Wolins

Department of Medicine, Washington University School of Medicine, St. Louis, MO 63110

Corresponding Author: nwolins{at}im.wustl.edu

We have developed a reliable, rapid, and economical assay for quantification of triacylglycerol (TG) in cells and animal tissues. In a few hours this assay quantifies microgram amounts of TG from tens or even hundreds of samples. The protocol includes an organic extraction to partition TG away from proteins and other hydrophilic molecules found in cells and tissues that may interfere with the colorimetric enzyme-linked TG detection method. In addition, this assay is economical as no expensive reagents, supplies or equipment is needed. Another benefit of this assay is that it does not require environmentally unfriendly halogenated solvents.


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