J. Lipid Res. Neurobiology of Lipids (ISSN1683-5506)
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A more recent version of this article appeared on May 1, 2008

Papers In Press, published online ahead of print February 13, 2008
J. Lipid Res., doi:10.1194/jlr.D800002-JLR200
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Submitted on January 2, 2008
Accepted on February 13, 2008

A rapid, microplate SNP genotype assay for the leptinob allele

Angie T. Oler and Alan D. Attie

Biochemistry, University of Wisconsin Madison, Madison, WI 53716

Corresponding Author: attie{at}biochem.wisc.edu

A nonsense mutation in the mouse leptin gene causes genetic obesity. Due to extensive research in the field of obesity, the use of leptinob mice is widespread. This mutation renders mice sterile, creating the need to breed heterozygous mice. For this reason, leptinob genotyping is necessary. To date, gel based assays are used for genotyping. Utilizing Invader Plus™ assay for SNP detection, we have developed a gel-free microplate SNP assay for genotyping leptinwt and leptinob alleles.


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