J. Lipid Res.
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

A more recent version of this article appeared on November 1, 2002

Papers In Press, published online ahead of print August 16, 2002
J. Lipid Res., doi:10.1194/jlr.M100441-JLR200
This Article
Right arrow All Versions of this Article:
M100441-JLR200v1
43/11/1846    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by van Greevenbroek, M. M. J.
Right arrow Articles by de Bruin, T. W. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by van Greevenbroek, M. M. J.
Right arrow Articles by de Bruin, T. W. A.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Submitted on December 27, 2001
Revised on June 6, 2002
Accepted on July 31, 2002

Familial combined hyperlipidemia plasma stimulates protein secretion by HepG2 cells: identification of fibronectin in the differential secretion proteome

M. M. J. van Greevenbroek, V. M. M-J Vermeulen, and T. W. A. de Bruin

Internal Medicine, Maastricht University, Maastricht 6200 MD

Corresponding Author: m.vangreevenbroek{at}intmed.unimaas.nl

This study evaluates whether soluble factors in plasma of Familial Combined Hyperlipidemia (FCHL) patients affect the secretion of hepatic proteins or lipoproteins. Cultured human hepatocytes, i.e. HepG2 cells, were incubated with fasting plasma (20% vol/vol in DMEM) from untreated FCHL patients or normolipidemic controls. Overall protein secretion was 10-15% higher after stimulation with FCHL plasma than with control plasma. FCHL plasma specifically induced secretion of 4 distinct proteins with estimated sizes of 240, 180, 120, and <40 kD on SDS gelelectrophoresis (p<0.001, p<0.006, p<0.002, p<0.02, respectively). The 240 kD protein in the secretion proteome was identified as fibronectin on mass spectrometry. Furthermore, plasma fibronectin concentrations were elevated in FCHL patients, confirming biological relevance of these data. Overall protein secretion correlated with triglyceride concentrations in the plasma samples studied (r=0.49, p<0.001). Specifically, incubation of HepG2 cells with VLDL+IDL fractions isolated from FCHL patients induced a higher protein secretion than lipoproteins isolated from controls (p<0.001). Plasma from both FCHL patients and controls induced apoB secretion by HepG2 cells, but there was no evidence of excess apoB production with FCHL plasma. In conclusion: FCHL plasma, and specifically FCHL-derived VLDL+IDL, stimulated excess secretion of several hepatic proteins, and one of those was identified as fibronectin. This specific response of hepatocytes to FCHL plasma justifies further study to the cellular mechanisms involved in this adaptation process.


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 J. Lipid Res.Home page
R. M. Crooke, M. J. Graham, K. M. Lemonidis, C. P. Whipple, S. Koo, and R. J. Perera
An apolipoprotein B antisense oligonucleotide lowers LDL cholesterol in hyperlipidemic mice without causing hepatic steatosis
J. Lipid Res., May 1, 2005; 46(5): 872 - 884.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
 All ASBMB Journals   Journal of Biological Chemistry 
 Molecular and Cellular Proteomics   ASBMB Today 
Copyright © 2002 by the American Society for Biochemistry and Molecular Biology.