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Papers In Press, published online ahead of print September 16, 2002
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Submitted on April 22, 2002
UPRES Lipides et Nutrition, Faculte des Sciences Gabriel, DIJON, Côte d'Or 21000
Corresponding Author: pclouet{at}u-bourgogne.fr
Conjugated linoleic acids (CLA) when added to diets in low amounts exert beneficial actions upon various cellular functions. Their effectiveness suggests that they should be maximally conserved and raises the question about their ability to be oxidised in mitochondria. Cis-9, trans-11-C18:2 (CLA1) and trans-10, cis-12-C18:2 (CLA2), the most widely used CLA isomers, were compared to cis-9, cis-12-C18:2 (linoleic acid ; LA) and cis-9-C16:1 (palmitoleic acid ; PA), as substrates for total fatty acid oxidation and for all the enzymatic steps required for the entry of fatty acids into the matrix in rat liver mitochondria. Oxygen consumption rate was lowest when CLA1 was used as a substrate with that on CLA2 being intermediate between it and the respiration on LA and PA. The order of the rate of oxidation of the radiolabeled fatty acids was PA >> LA > CLA2 > CLA1. Activation by CoA and transesterification to acylcarnitines of all the octadecadienoic acids were very similar, while uptake across the mitochondrial inner membranes of CLA1 and, to a lesser extent, of CLA2 was greater than that of LA or PA. Prior oxidation of CLA1 or CLA2 made re-isolated mitochondria much less capable of oxidising PA or LA under carnitine-dependent conditions, but did not alter the carnitine-independent oxidation of octanoic acid. Therefore in addition to their poor oxidisability, the CLA isomers studied appeared capable of interfering with the oxidation of usual fatty acids at a step close to the beginning of the ß-oxidative cycle.
Revised on July 13, 2002
Accepted on September 10, 2002
Conjugated linoleic acid isomers in mitochondria : Evidence for an alteration of fatty acid oxidation
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