J. Lipid Res. Neurobiology of Lipids (ISSN1683-5506)
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

A more recent version of this article appeared on December 1, 2002

Papers In Press, published online ahead of print September 16, 2002
J. Lipid Res., doi:10.1194/jlr.M200241-JLR200
This Article
Right arrow Full Text (Accepted Manuscript)
Right arrow All Versions of this Article:
M200241-JLR200v1
43/12/2042    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Cowart, L. A.
Right arrow Articles by Hannun, Y. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Cowart, L. A.
Right arrow Articles by Hannun, Y. A.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Submitted on June 20, 2002
Revised on August 29, 2002
Accepted on September 3, 2002

Structural determinants of sphingolipid recognition by commercially available anti-ceramide antibodies

L. Ashley Cowart, Zdzislaw Szulc, Alicja Bielawska, and Yusuf A. Hannun

Biochemistry and Molecular Biology, Medical University of South Carolina, Charleston, SC

Corresponding Author: cowartL{at}musc.edu

The sphingolipid mediator ceramide is involved in cellular processes such as apoptosis, differentiation, responses to cytokines, and stress responses. Experimental evidence suggests that the intracellular location of ceramide may be a key factor in determining its ultimate cellular effects. One approach to ceramide localization is the use of recently developed anti-ceramide antibodies for immunocytochemical studies. Two such commercial preparations are now available; we sought to compare and contrast their specificity for ceramide and/or other cellular lipids. By using lipid overlay assays and a diverse panel of sphingolipids, we were able to delineate the specificity and thus, the utility of these reagents. Our results indicate that one of these anti-ceramide preparations is quite specific for ceramide and dihydroceramide, whereas the other preparation recognizes dihydroceramide, phosphatidylcholine (PC), and sphingomyelin (SM). Furthermore, through the use of chemically modified ceramides in similar assays, we were able to determine some structural determinants of lipid recognition by both of these reagents.


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 J. Biol. Chem.Home page
S. DeMorrow, S. Glaser, H. Francis, J. Venter, B. Vaculin, S. Vaculin, and G. Alpini
Opposing Actions of Endocannabinoids on Cholangiocarcinoma Growth: RECRUITMENT OF Fas AND Fas LIGAND TO LIPID RAFTS
J. Biol. Chem., April 27, 2007; 282(17): 13098 - 13113.
[Abstract] [Full Text] [PDF]

Home page
 J. Lipid Res.Home page
K. Krishnamurthy, S. Dasgupta, and E. Bieberich
Development and characterization of a novel anti-ceramide antibody
J. Lipid Res., April 1, 2007; 48(4): 968 - 975.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
K. Krishnamurthy, G. Wang, J. Silva, B. G. Condie, and E. Bieberich
Ceramide Regulates Atypical PKC{zeta}/{lambda}-mediated Cell Polarity in Primitive Ectoderm Cells: A NOVEL FUNCTION OF SPHINGOLIPIDS IN MORPHOGENESIS
J. Biol. Chem., February 2, 2007; 282(5): 3379 - 3390.
[Abstract] [Full Text] [PDF]

Home page
 FASEB J.Home page
X. L. Guan, X. He, W.-Y. Ong, W. K. Yeo, G. Shui, and M. R. Wenk
Non-targeted profiling of lipids during kainate-induced neuronal injury
FASEB J, June 1, 2006; 20(8): 1152 - 1161.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
C.-s. Liang, W. Mao, C. Iwai, S. Fukuoka, and S. Y. Stevens
Cardiac sympathetic neuroprotective effect of desipramine in tachycardia-induced cardiomyopathy
Am J Physiol Heart Circ Physiol, March 1, 2006; 290(3): H995 - H1003.
[Abstract] [Full Text] [PDF]

Home page
 J. Leukoc. Biol.Home page
L. Franchi, F. Malisan, B. Tomassini, and R. Testi
Ceramide catabolism critically controls survival of human dendritic cells
J. Leukoc. Biol., January 1, 2006; 79(1): 166 - 172.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
G. Wang, J. Silva, K. Krishnamurthy, E. Tran, B. G. Condie, and E. Bieberich
Direct Binding to Ceramide Activates Protein Kinase C{zeta} before the Formation of a Pro-apoptotic Complex with PAR-4 in Differentiating Stem Cells
J. Biol. Chem., July 15, 2005; 280(28): 26415 - 26424.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
 All ASBMB Journals   Journal of Biological Chemistry 
 Molecular and Cellular Proteomics   ASBMB Today 
Copyright © 2002 by the American Society for Biochemistry and Molecular Biology.