| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
|
|
|
|||||||
|
|||||||||
Papers In Press, published online ahead of print November 4, 2002
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Physiology & Biophysics, University of Medicine & Dentistry of New Jersey, Piscataway, New Jersey 08854
Corresponding Author: lenard{at}umdnj.edu
Caenorhabditis elegans requires dietary sterol, usually supplied as cholesterol. Sterols are enzymatically modified, however, and many different sterols can meet the requirement. Sterol deprivation decreased brood size and adult growth in the first generation, and completely, but reversibly, arrested growth during larval development in the second. After one generation of sterol deprivation addition of cholesterol at concentrations as low as 10 ng/ml allowed delayed laying of a few eggs, but 100 ng/ml was required for stimulation of growth. C. elegans is known to synthesize two unusual 4
Revised on October 15, 2002
Accepted on November 4, 2002
Sterol effects and sites of sterol accumulation in Caenorhabditis elegans: developmental requirement for 4
-methyl sterols
-methyl sterols (4MSs) in substantial amounts from supplied sterols. We find that 4MSs supported only limited growth as the sole sterol. However, addition of only 10 ng of cholesterol to 1000 ng of 4MS could restore full growth and egg-laying. This synergism suggests that both 4MS and unmethylated sterols, acting through different pathways, are required for development. Sterols were found by filipin staining to accumulate in only a few specific cells: the excretory gland cell, two amphid socket cells, two phasmid socket cells and, in the male, spicule socket cells. Sterols were also present in the pharynx and in the intestine of feeding animals, in a proximal-to-distal gradient. This uneven sterol distribution, the low concentration requirements, and the effects of 4MSs argues that sterols are unlikely to be used for bulk structural modification of cell membranes, but are more likely required as hormone precursors and/or developmental effectors.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  S. Desnoyers, P.-G. Blanchard, J.-F. St-Laurent, S. N Gagnon, D. L Baillie, and V. Luu-The Caenorhabditis elegans LET-767 is able to metabolize androgens and estrogens and likely shares common ancestor with human types 3 and 12 17{beta}-hydroxysteroid dehydrogenases J. Endocrinol., November 1, 2007; 195(2): 271 - 279. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. R. Glodowski, C. C.-H. Chen, H. Schaefer, B. D. Grant, and C. Rongo RAB-10 Regulates Glutamate Receptor Recycling in a Cholesterol-dependent Endocytosis Pathway Mol. Biol. Cell, November 1, 2007; 18(11): 4387 - 4396. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Merris, T. Wang, P. Soteropoulos, and J. Lenard Differential gene expression of Caenorhabditis elegans grown on unmethylated sterols or 4{alpha}-methylsterols J. Lipid Res., May 1, 2007; 48(5): 1159 - 1166. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 O. Zamir and M. P. Charlton Cholesterol and synaptic transmitter release at crayfish neuromuscular junctions J. Physiol., February 15, 2006; 571(1): 83 - 99. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Novillo, S.-J. Won, C. Li, and I. P. Callard Changes in Nuclear Receptor and Vitellogenin Gene Expression in Response to Steroids and Heavy Metal in Caenorhabditis elegans Integr. Comp. Biol., January 1, 2005; 45(1): 61 - 71. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Li, G. Brown, M. Ailion, S. Lee, and J. H. Thomas NCR-1 and NCR-2, the C. elegans homologs of the human Niemann-Pick type C1 disease protein, function upstream of DAF-9 in the dauer formation pathways Development, November 15, 2004; 131(22): 5741 - 5752. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Merris, J. Kraeft, G. S. Tint, and J. Lenard Long-term effects of sterol depletion in C. elegans: sterol content of synchronized wild-type and mutant populations J. Lipid Res., November 1, 2004; 45(11): 2044 - 2051. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. P. Grigorenko, Y. K. Moliaka, M. C. Soto, C. C. Mello, and E. I. Rogaev The Caenorhabditis elegans IMPAS gene, imp-2, is essential for development and is functionally distinct from related presenilins PNAS, October 12, 2004; 101(41): 14955 - 14960. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Nehrke A Reduction in Intestinal Cell pHi Due to Loss of the Caenorhabditis elegans Na+/H+ Exchanger NHX-2 Increases Life Span J. Biol. Chem., November 7, 2003; 278(45): 44657 - 44666. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B.-K. Choi, D. J. Chitwood, and Y.-K. Paik Proteomic Changes during Disturbance of Cholesterol Metabolism by Azacoprostane Treatment in Caenorhabditis elegans Mol. Cell. Proteomics, October 1, 2003; 2(10): 1086 - 1095. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
| All ASBMB Journals | Journal of Biological Chemistry |
| Molecular and Cellular Proteomics | ASBMB Today |
