| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
|
|
|
|||||||
|
|||||||||
Papers In Press, published online ahead of print November 16, 2002
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Laboratory of Molecular Liver Cell Biology, Free University Brussels, Brussel 1090
Corresponding Author: khellem{at}minf.vub.ac.be
Activation of cultured hepatic stellate cells correlated with an enhanced expression of proteins involved in uptake and storage of fatty acids (FA translocase CD36, Acyl-CoA synthetase 2) and retinol (cellular retinol binding protein type I, CRBP-I lecithin:retinol acyltransferases, LRAT). The increased expression of CRBP-I and LRAT during HSC activation, both involved in retinol esterification, was in contrast with the simultaneous depletion of their typical lipid/vitamin A reserves. Since HSC express high levels of peroxisome proliferator activated receptor
Revised on November 13, 2002
Accepted on November 14, 2002
PPAR
regulates vitamin A-metabolism related gene expression in hepatic stellate cells undergoing activation
(PPAR), which become further induced during transition into the activated phenotype, we investigated the potential role of PPARin the regulation of these changes. Administration of L165041, a PPAR-specific agonist, further induced the expression of CD36, B-FABP, CRBP-I and LRAT, whereas their expression was inhibited by antisense PPAR mRNA. PPAR:RXR dimers bound to CRBP-I promoter sequences. Our observations suggest that PPAR regulates the expression of these genes, and thus could play an important role in vitamin A storage. In vivo, we observed a striking association between the enhanced expression of PPAR and CRBP-I in activated myofibroblast like-HSC and the manifestation of vitamin A autofluorescent droplets in the fibrotic septa after injury with CCl4 or CCl4 in combination with retinol.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  J. H. Cheng, H. She, Y.-P. Han, J. Wang, S. Xiong, K. Asahina, and H. Tsukamoto Wnt antagonism inhibits hepatic stellate cell activation and liver fibrosis Am J Physiol Gastrointest Liver Physiol, January 1, 2008; 294(1): G39 - G49. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. R. Moise, M. Golczak, Y. Imanishi, and K. Palczewski Topology and Membrane Association of Lecithin: Retinol Acyltransferase J. Biol. Chem., January 19, 2007; 282(3): 2081 - 2090. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 X.-H. Tang, M.-J. Suh, R. Li, and L. J. Gudas Cell proliferation inhibition and alterations in retinol esterification induced by phytanic acid and docosahexaenoic acid J. Lipid Res., January 1, 2007; 48(1): 165 - 176. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 I A Leclercq, C Sempoux, P Starkel, and Y Horsmans Limited therapeutic efficacy of pioglitazone on progression of hepatic fibrosis in rats Gut, July 1, 2006; 55(7): 1020 - 1029. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. A. Peraza, A. D. Burdick, H. E. Marin, F. J. Gonzalez, and J. M. Peters The Toxicology of Ligands for Peroxisome Proliferator-Activated Receptors (PPAR) Toxicol. Sci., April 1, 2006; 90(2): 269 - 295. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. She, S. Xiong, S. Hazra, and H. Tsukamoto Adipogenic Transcriptional Regulation of Hepatic Stellate Cells J. Biol. Chem., February 11, 2005; 280(6): 4959 - 4967. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
| All ASBMB Journals | Journal of Biological Chemistry |
| Molecular and Cellular Proteomics | ASBMB Today |
