| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
|
|
|
|||||||
|
|||||||||
Papers In Press, published online ahead of print January 1, 2003
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Ob/Gyn and Biochemistry, University of Western Ontario, London, ON N6A 5A5
Corresponding Author: fpossmay{at}uwo.ca
Pulmonary surfactant is a lipid:protein complex which contains dipalmitoylphosphatidylcholine (DPPC) as the major component. Recent studies indicate adsorbed surfactant films consist of a surface monolayer and a monolayer-associated reservoir. It has been hypothesized that the reservoir is functionally contiguous with the monolayer, and that the monolayer may be enriched in DPPC relative to bulk phase surfactant. We investigated the lipid composition relationship between the monolayer and its reservoir using a filter paper-supported wet bridge to transfer film from adsorbing dishes to clean surfaces on spreading dishes. The spreading films appeared to form monolayers on the spreading dish surface. The studies employed BLES(chol) (bovine lipid extract surfactant containing cholesterol) containing [3H]DPPC and either [14C] radiolabelled palmitoyl, oleoylphosphatidylcholine (POPC); [14C]dipalmitoylphosphatidylglycerol (DPPG); [14C]palmitoyl, oleoylphosphatidylglycerol (POPG); or [14C]cholesterol. Radiolabelled PGs were prepared using phospholipase D. The studies demonstrated that the [3H] DPPC/[14C] POPC ratios were the same in the prepared BLES dispersions as in Langmuir-Blodgett films, indicating a lack of selectivity for DPPC in film formation. Furthermore, [3H]/[14C] isotopic ratios were observed with DPPC and either [14C] labelled POPC, DPPG, POPG, or cholesterol in the original dispersions, the bulk phase of adsorption dish D1, and monolayers recovered from spreading dish D2. These relationships remained unperturbed with 2.0-fold or more increases in bulk concentrations in D1 and a 10-fold or more variation in D1:D2 surface areas. These results indicate adsorbed surfactant monolayers and their associated reservoirs possess similar lipid compositions and argue against selective adsorption of DPPC.
Revised on December 13, 2002
Accepted on December 18, 2002
Lipid compositional analysis of pulmonary surfactant monolayers and monolayer-associated reservoirs
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  S. Baoukina, L. Monticelli, M. Amrein, and D. P. Tieleman The Molecular Mechanism of Monolayer-Bilayer Transformations of Lung Surfactant from Molecular Dynamics Simulations Biophys. J., December 1, 2007; 93(11): 3775 - 3782. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Z. Leonenko, S. Gill, S. Baoukina, L. Monticelli, J. Doehner, L. Gunasekara, F. Felderer, M. Rodenstein, L. M. Eng, and M. Amrein An Elevated Level of Cholesterol Impairs Self-Assembly of Pulmonary Surfactant into a Functional Film Biophys. J., July 15, 2007; 93(2): 674 - 683. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Y. Zuo and F. Possmayer How does pulmonary surfactant reduce surface tension to very low values? J Appl Physiol, May 1, 2007; 102(5): 1733 - 1734. [Full Text] [PDF]
|
|
|
|
 |
|
 H. Nakanishi, H. Shindou, D. Hishikawa, T. Harayama, R. Ogasawara, A. Suwabe, R. Taguchi, and T. Shimizu Cloning and Characterization of Mouse Lung-type Acyl-CoA:Lysophosphatidylcholine Acyltransferase 1 (LPCAT1): EXPRESSION IN ALVEOLAR TYPE II CELLS AND POSSIBLE INVOLVEMENT IN SURFACTANT PRODUCTION J. Biol. Chem., July 21, 2006; 281(29): 20140 - 20147. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Lee, S. K. Kandasamy, and R. G. Larson Molecular Dynamics Simulations of the Anchoring and Tilting of the Lung-Surfactant Peptide SP-B1-25 in Palmitic Acid Monolayers Biophys. J., December 1, 2005; 89(6): 3807 - 3821. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Bernardino de la Serna, J. Perez-Gil, A. C. Simonsen, and L. A. Bagatolli Cholesterol Rules: DIRECT OBSERVATION OF THE COEXISTENCE OF TWO FLUID PHASES IN NATIVE PULMONARY SURFACTANT MEMBRANES AT PHYSIOLOGICAL TEMPERATURES J. Biol. Chem., September 24, 2004; 279(39): 40715 - 40722. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Frisk, J. R. Schurr, G. Wang, D. C. Bertucci, L. Marrero, S. H. Hwang, D. J. Hassett, and M. J. Schurr Transcriptome Analysis of Pseudomonas aeruginosa after Interaction with Human Airway Epithelial Cells Infect. Immun., September 1, 2004; 72(9): 5433 - 5438. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. K. Panda, K. Nag, R. R. Harbottle, K. Rodriguez-Capote, R. A. W. Veldhuizen, N. O. Petersen, and F. Possmayer Effect of Acute Lung Injury on Structure and Function of Pulmonary Surfactant Films Am. J. Respir. Cell Mol. Biol., May 1, 2004; 30(5): 641 - 650. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
| All ASBMB Journals | Journal of Biological Chemistry |
| Molecular and Cellular Proteomics | ASBMB Today |
