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Papers In Press, published online ahead of print March 1, 2003
J. Lipid Res., doi:10.1194/jlr.M200382-JLR200
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Centre de Biochimie, Faculté des Sciences, CNRS UMR 6543, Nice 06108
Corresponding Author: amri{at}unice.fr
Exposure of preadipocytes to long chain fatty acids induced expression of several markers of adipocyte differentiation. In an attempt to identify novel genes and proteins that are regulated by fatty acids in preadipocytes, we performed a substractive hybridization screening and identified PTX3, a protein of the pentraxin family. PTX3 mRNA expression is transient during adipocyte differentiation of clonal cell lines and is absent in fully differentiated cells. Stable overexpression of PTX3 in preadipocytes has no effect on adipocyte differentiation. In line with this, PTX3 mRNA is expressed in the stromal-vascular fraction of adipose tissue, but not in the adipocyte fraction. However, in 3T3-F442A adipocytes, PTX3 gene can be re-induced by TNFá in a dose dependant manner. This effect is accompanied by PTX3 protein secretion from both 3T3-F442A adipocytes and explants of mouse adipose tissue. PTX3 mRNA levels are found to be higher in adipose tissue of genetically-obese mice versus control mice, consistent with their increased TNFá levels. In conclusion, PTX3 appears as a TNFá-induced protein which provides a new link between chronic low-level inflammatory state and obesity.
Revised on February 13, 2003
Accepted on February 24, 2003
Characterization of the long pentraxin PTX3 as a TNF
-induced, secreted protein of adipose cells
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