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Papers In Press, published online ahead of print February 1, 2003
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INSERM U 499, Faculté LAENNEC, LYON 69008
Corresponding Author: beylot{at}laennec.univ-lyon1.fr
Adipose tissue de novo lipogenesis (DNL) is considered as minor in humans. However recent studies suggested that it could contribute to fat stores in subjects massively overfed with carbohydrates. We assessed the contributions of human liver and adipose tissue DNL to triacylglycerol (TAG) synthesis and their response to orally ingested carbohydrates. Volunteers were fed either a two week high energy, high carbohydrate diet (HC, n=5) or a normocaloric diet (NC, n=10). NC subjects remained in the fasting state (study 1, n=5) or received oral glucose (study 2, n=5) throughout the 12 hours of the test. HC subjects remained in the fasting state (study 3). They ingested deuterated water and [U-13C]-acetate to trace lipogenesis. Fatty acid synthase (FAS), acetyl-CoA carboxylase 1 (ACC1) and SREBP-1c mRNA were measured in adipose tissue. Plasma TAG-FA were labeled by 13C and deuterium showing active liver lipogenesis, which was stimulated (p<0.05) by oral glucose and the HC diet. Adipose tissue TAG had no detectable 13C enrichment in any test, showing no significant incorporation of TAG-FA provided by liver lipogenesis. Deuterium enrichments were found in adipose TAG in all tests showing active DNL in situ. However rough quantitative estimates showed that adipose DNL was minimal (<1g) and poorly stimulated by oral glucose or the HC diet. FAS, ACC1 and SREBP-1c mRNA levels were not increased by the HC diet. Conclusion : adipose DNL is active in humans, but contributes little to TAG stores and is less responsive than liver DNL to stimulation by carbohydrates.
Revised on January 16, 2003
Accepted on January 16, 2003
Differences in the regulation of adipose tissue and liver lipogenesis by carbohydrates in humans
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