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A more recent version of this article appeared on May 1, 2003

Papers In Press, published online ahead of print March 1, 2003
J. Lipid Res., doi:10.1194/jlr.M200478-JLR200
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Submitted on December 19, 2002
Revised on February 25, 2003
Accepted on February 25, 2003

Effect of ursodeoxycholic acid on cholesterol absorption and metabolism in humans

Laura A. Woollett, Donna D. Buckley, Lihang Yao, Peter J.H. Jones, Norman A. Granholm, Elizabeth A. Tolley, and James E. Heubi

Pediatrics, Children's Hospital Medical Center, Cincinnati, OH 45229

Corresponding Author: james.heubi{at}chmcc.org

Qualitative and quantitative changes in intralumenal bile acid composition may alter cholesterol absorption and synthesis and LDL-receptor expression. In a randomized crossover design outpatient study, 12 adults aged 24-36 years took 15 mg/kg/day ursodeoxycholic acid (UDCA) or no bile acid supplement (control) for 20 days while being fed a controlled diet (AHA Step II). A liquid meal of defined composition was then given and lumenal samples collected. Cholesterol absorption and cholesterol fractional synthetic rate (FSR) were assessed by stable isotopic methods. With UDCA treatment, bile was enriched significantly (p<0.0001) to 40.6+/-2.6% (mean +/-S.E.M.) compared to 2.2+/-2.6% for controls. Regardless, plasma total, HDL-and LDL-cholesterol was unchanged with UDCA treatment. Intralumenal cholesterol solubilized in the aqueous phase during the entire collection was decreased (p=0.012) in UDCA-treated (101.0 +/-7.2 mg/ml/120 min) compared to controls (132.5+/-7.2 mg/ml/120 min.). Percent micellar cholesterol was increased in UDCA treated vs. controls after meal ingestion. No changes were found in cholesterol absorption, FSR or LDL-receptor mRNA with UDCA treatment compared to controls. Thus, despite marked enrichment in lumenal bile with UDCA and decreased cholesterol solubilization, no differences in cholesterol absorption or metabolism are found when diet and genetic differences in absorption are carefully controlled.


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L A Woollett, Y Wang, D D Buckley, L Yao, S Chin, N Granholm, P J H Jones, K D R Setchell, P Tso, and J E Heubi
Micellar solubilisation of cholesterol is essential for absorption in humans
Gut, February 1, 2006; 55(2): 197 - 204.
[Abstract] [Full Text] [PDF]


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