| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
|
|
|
|||||||
|
|||||||||
Papers In Press, published online ahead of print September 16, 2003
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Research, Children's Hospital Oakland, Oakland, CA 94609
Corresponding Author: jsaba{at}chori.org
Sphingolipid metabolites function as signaling molecules in mammalian cells, influencing cell proliferation, migration and death. Recently, sphingolipid signaling has been implicated in the regulation of developmental processes in Drosophila melanogaster. However, biochemical analysis of endogenous Drosophila sphingoid bases has not been reported. In this study, a rapid HPLC based method was developed for the analysis of free sphingoid bases endogenous to Drosophila. Four molecular species of endogenous free sphingoid bases were observed in adult flies and identified as C14 and C16 sphingosine and C14 and C16 dihydrosphingosine. The C14 molecular species were the most prevalent, accounting for approximately 94 percent of the total free sphingoid bases in adult wild type flies. A sphingosine kinase mutant demonstrated significant accumulation of all four sphingoid bases, whereas a serine palmitoyltransferase mutant demonstrated low but detectable levels. When endogenous sphingoid bases were evaluated at different stages of development, the observed ratio of sphingosine to dihydrosphingosine increased significantly from early embryos to adulthood. Throughout development, this ratio was significantly lower in the sphingosine kinase mutant as compared to the wild type. This is the first report describing analysis of free C14 and C16 sphingoid bases from Drosophila. The biochemical characterization of these lipids from mutant models of sphingolipid metabolism should greatly facilitate the analysis of the biological significance of these signaling molecules.
Revised on September 2, 2003
Accepted on September 12, 2003
Characterization of free endogenous C14 and C16 sphingoid bases from Drosophila melanogaster
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  S. T. Pruett, A. Bushnev, K. Hagedorn, M. Adiga, C. A. Haynes, M. C. Sullards, D. C. Liotta, and A. H. Merrill Jr. Thematic Review Series: Sphingolipids. Biodiversity of sphingoid bases ("sphingosines") and related amino alcohols J. Lipid Res., August 1, 2008; 49(8): 1621 - 1639. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Fyrst, X. Zhang, D. R. Herr, H. S. Byun, R. Bittman, V. H. Phan, G. L. Harris, and J. D. Saba Identification and characterization by electrospray mass spectrometry of endogenous Drosophila sphingadienes J. Lipid Res., March 1, 2008; 49(3): 597 - 606. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Kohno, M. Momoi, M. L. Oo, J.-H. Paik, Y.-M. Lee, K. Venkataraman, Y. Ai, A. P. Ristimaki, H. Fyrst, H. Sano, et al. Intracellular role for sphingosine kinase 1 in intestinal adenoma cell proliferation. Mol. Cell. Biol., October 1, 2006; 26(19): 7211 - 7223. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Bandhuvula, Y. Y. Tam, B. Oskouian, and J. D. Saba The Immune Modulator FTY720 Inhibits Sphingosine-1-phosphate Lyase Activity J. Biol. Chem., October 7, 2005; 280(40): 33697 - 33700. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Q. Jiang, J. Wong, H. Fyrst, J. D. Saba, and B. N. Ames {gamma}-Tocopherol or combinations of vitamin E forms induce cell death in human prostate cancer cells by interrupting sphingolipid synthesis PNAS, December 21, 2004; 101(51): 17825 - 17830. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
| All ASBMB Journals | Journal of Biological Chemistry |
| Molecular and Cellular Proteomics | ASBMB Today |
