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Papers In Press, published online ahead of print May 16, 2003
J. Lipid Res., doi:10.1194/jlr.M300022-JLR200
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Biochemical Physiology, Utrecht University, Utrecht, Utrecht NL-3584 CH
Corresponding Author: d.vanhoof{at}bio.uu.nl
High-density lipophorin (HDLp) in the circulation of insects is able to selectively deliver lipids to target tissues in a non-endocytic manner. In Locusta migratoria, a member of the low-density lipoprotein (LDL) receptor (LDLR) family has been identified and shown to mediate endocytosis of HDLp in mammalian cells transfected with the cDNA of this receptor. This insect lipophorin receptor (iLR) is temporally expressed in fat body tissue of young adult as well as larval locusts as shown by Western blot analysis. Fluorescence microscopy revealed that fat body cells internalize fluorescently-labeled HDLp and human receptor-associated protein (RAP) only when iLR is expressed. Expression of iLR is down-regulated on the fourth day after an ecdysis. Consequently, HDLp is no longer internalized. By starving adult locusts immediately after ecdysis, we were able to prolong iLR expression. In addition, expression of the receptor was induced by starving adults after down-regulation of iLR. These results suggest that iLR mediates endocytosis of HDLp in fat body cells, and that expression of iLR is regulated by the demand of fat body tissue for lipids.
Revised on April 22, 2003
Accepted on May 13, 2003
Lipophorin receptor-mediated lipoprotein endocytosis in insect fat body cells
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