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A more recent version of this article appeared on June 1, 2003

Papers In Press, published online ahead of print March 16, 2003
J. Lipid Res., doi:10.1194/jlr.M300033-JLR200
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Submitted on January 21, 2003
Revised on March 10, 2003
Accepted on March 10, 2003

Visualizing caveolin-1 and HDL in cholesterol-loaded aortic endothelial cells

Wei Ting Chao, Seng Sheen Fan, Jan Kan Chen, and Vie Cheng Yang

Department of Biology and Life Science Research Center, Tunghai university, Taichung, Taiwan 407

Corresponding Author: vcyang{at}mail.thu.edu.tw

Caveolae are vesicular invaginations of the plasma membranes that regulate signal transduction and transcytosis, as well as cellular cholesterol homeostasis. Our previous studies indicated that the removal of cholesterol from aortic endothelial cells and smooth muscle cells in the presence of HDL is associated with plasmalemmal invaginations and plasmalemmal vesicles. The goal of the present study was to investigate the location and distribution of caveolin-1, the main structural protein component of caveolae, in cholesterol-loaded aortic endothelial cells after HDL incubation. Confocal microscopic analysis demonstrated that the caveolin-1 appeared to colocalize with HDL-DiI conjugates on the cell surface. No free HDL-DiI conjugates were revealed in the cytoplasm. Immunoelectron microscopy further demonstrated that caveolin-1-gold (15 nm) conjugates colocalized with HDL-gold (10 nm) conjugates in the plasmalemmal invaginations. These morphological results indicated that caveolae are the major membrane domains facilitating the transport of excess cholesterol to HDL on the cell surface of aortic endothelial cells.


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Y. Fu, A. Hoang, G. Escher, R. G. Parton, Z. Krozowski, and D. Sviridov
Expression of Caveolin-1 Enhances Cholesterol Efflux in Hepatic Cells
J. Biol. Chem., April 2, 2004; 279(14): 14140 - 14146.
[Abstract] [Full Text] [PDF]


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