J. Lipid Res. Did you know there is a large type edition? Click here.
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

A more recent version of this article appeared on June 1, 2003

Papers In Press, published online ahead of print April 1, 2003
J. Lipid Res., doi:10.1194/jlr.M300037-JLR200
This Article
Right arrow Full Text (Accepted Manuscript)
Right arrow All Versions of this Article:
M300037-JLR200v1
44/6/1241    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Duan, R.-D.
Right arrow Articles by Nilsson, A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Duan, R.-D.
Right arrow Articles by Nilsson, A.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Submitted on January 27, 2003
Revised on March 14, 2003
Accepted on March 20, 2003

Purification, localization and expression of human intestinal alkaline sphingomyelinase

Rui-Dong Duan, Yajun Cheng, Gert Hansen, Erik Hertervig, Jian-Jun Liu, Ingvar Syk, Hans Sjöström, and Åke Nilsson

Biomedical Center, B11, Gastroenterology Lab, Lund S-221 84

Corresponding Author: rui-dong.duan{at}med.lu.se

Sphingomyelin metabolism in the gut may have an impact on colon cancer development. In this study we purified alkaline sphingomyelinase (alk-SMase) from human intestinal content, and studied its location in the mucosa, expression in colon cancer and function on colon cancer cells. The enzyme was purified by a series of chromatographies. Its molecular mass is 60 kDa, optimal pH is 8.5 and isoelectric point is 6.6. Under optimal conditions, 1 mg enzyme hydrolyzed 11 mM SM per hour. The properties of the enzyme are similar to those of rat intestinal alkaline SMase but not to those of bacterial neutral SMase. Immunogold electronmicroscopy identified the enzyme on the microvillar membrane, in endosome-like structures, and in the Golgi complexes of human enterocytes. The expression and the activity of the enzyme were decreased in parallel in human colon cancer tissues compared to the adjacent normal tissue. The enzyme inhibited DNA biosynthesis and cell proliferation dose-dependently and caused a reduction of sphingomyelin in HT29 cells. Conclusion. Intestinal alk-SMase is localized in the enterocytes, down regulated in human colon cancer and may have antiproliferative effects on colon cancer cells.


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 Anesth. Analg.Home page
K.-X. Liu, S.-Q. Chen, W.-Q. Huang, Y.-S. Li, M. G. Irwin, and Z. Xia
Propofol Pretreatment Reduces Ceramide Production and Attenuates Intestinal Mucosal Apoptosis Induced by Intestinal Ischemia/Reperfusion in Rats
Anesth. Analg., December 1, 2008; 107(6): 1884 - 1891.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
T. Yabu, S. Imamura, M. Yamashita, and T. Okazaki
Identification of Mg2+-dependent Neutral Sphingomyelinase 1 as a Mediator of Heat Stress-induced Ceramide Generation and Apoptosis
J. Biol. Chem., October 31, 2008; 283(44): 29971 - 29982.
[Abstract] [Full Text] [PDF]

Home page
 J. Lipid Res.Home page
A. Nilsson and R.-D. Duan
Absorption and lipoprotein transport of sphingomyelin
J. Lipid Res., January 1, 2006; 47(1): 154 - 171.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Epidemiol. Biomarkers Prev.Home page
L. Di Marzio, A. Di Leo, B. Cinque, D. Fanini, A. Agnifili, P. Berloco, M. Linsalata, D. Lorusso, M. Barone, C. De Simone, et al.
Detection of Alkaline Sphingomyelinase Activity in Human Stool: Proposed Role as a New Diagnostic and Prognostic Marker of Colorectal Cancer
Cancer Epidemiol. Biomarkers Prev., April 1, 2005; 14(4): 856 - 862.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
J. Wu, F. Liu, A. Nilsson, and R.-D. Duan
Pancreatic trypsin cleaves intestinal alkaline sphingomyelinase from mucosa and enhances the sphingomyelinase activity
Am J Physiol Gastrointest Liver Physiol, November 1, 2004; 287(5): G967 - G973.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
M. Olsson, R.-D. Duan, L. Ohlsson, and A. Nilsson
Rat intestinal ceramidase: purification, properties, and physiological relevance
Am J Physiol Gastrointest Liver Physiol, October 1, 2004; 287(4): G929 - G937.
[Abstract] [Full Text] [PDF]

Home page
 CarcinogenesisHome page
J. Wu, Y. Cheng, A. Nilsson, and R.-D. Duan
Identification of one exon deletion of intestinal alkaline sphingomyelinase in colon cancer HT-29 cells and a differentiation-related expression of the wild-type enzyme in Caco-2 cells
Carcinogenesis, August 1, 2004; 25(8): 1327 - 1333.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
R.-D. Duan, T. Bergman, N. Xu, J. Wu, Y. Cheng, J. Duan, S. Nelander, C. Palmberg, and A. Nilsson
Identification of Human Intestinal Alkaline Sphingomyelinase as a Novel Ecto-enzyme Related to the Nucleotide Phosphodiesterase Family
J. Biol. Chem., October 3, 2003; 278(40): 38528 - 38536.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
 All ASBMB Journals   Journal of Biological Chemistry 
 Molecular and Cellular Proteomics   ASBMB Today 
Copyright © 2003 by the American Society for Biochemistry and Molecular Biology.