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A more recent version of this article appeared on June 1, 2003

Papers In Press, published online ahead of print April 1, 2003
J. Lipid Res., doi:10.1194/jlr.M300090-JLR200
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Submitted on February 25, 2003
Revised on March 18, 2003
Accepted on March 19, 2003

Measurement of fasting serum apolipoprotein B-48 levels in normolipidemic and hyperlipidemic subjects by ELISA

Naohiko Sakai, Yoshiaki Uchida, Koji Ohashi, Toshiyuki Hibuse, Yasuhiko Saika, Yoshiaki Tomari, Shinji Kihara, Hisatoyo Hiraoka, Tadashi Nakamura, Satoru Ito, Shizuya Yamashita, and Yuji Matsuzawa

Department of Internal Medicine and Molecular Science, Osaka University, Suita, Osaka 565-0871

Corresponding Author: naosakai{at}imed2.med.osaka-u.ac.jp

The present study was designed to evaluate the metabolism of chylomicron and chylomicron remnants, by measuring serum apo B-48 levels in 335 normolipidemic and 253 hyperlipidemic subjects, using a novel ELISA system. The distribution of fasting serum apo B-48 levels in normolipidemic subjects varied widely, ranging from less than 1 to over 24 µg/ml (mean, 5.2 ± 3.8 µg/ml, SD, median, 3.9 µg/ml, and 95 percentile was less than 13 µg/ml, 1.3 mg/dl). Serum apo B-48 levels correlated with serum triglyceride concentrations (r=0.45, p<0.001), but not with total cholesterol levels. Serum apo B-48 levels were 7 to 18 times higher in patients with type I, type V and type III hyperlipidemia, and only slightly higher in patients with type IIa, type IIb and type IV hyperlipidemia, compared with normolipidemic subjects. The calculated B-48/TG ratio was elevated only in patients with dysbetalipoproteinemia (apo E2/2 phenotype). In normolipidemic subjects, oral fat loading resulted in about two-fold increase in serum apo B-48 levels with a peak level recorded at 3-4 hour postloading and then returned to the baseline level within 6 hours. On the other hand, in patients with dysbetalipoproteinemia, serum apo B-48 levels did not change considerably even in the presence of prolonged rise in TG concentrations. Our results indicate that serum apo B-48 is a very useful parameter for evaluating lipoprotein metabolism in exogenous pathway. Measurement of fasting serum apo B-48 levels could potentially be used as a simple tool to identify postprandial hyperlipidemia, an important coronary risk factor.


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