| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
|
|
|
|||||||
|
|||||||||
Papers In Press, published online ahead of print May 16, 2003
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Institute for Nutrition Research, University of Oslo, Oslo, Blindern 0316
Corresponding Author: h.i.nebb{at}basalmed.uio.no
A group of polyunsaturated fatty acids called conjugated linoleic acids (CLAs) are found in ruminant products, where the most common isomers are cis9, trans11 and trans10, cis12 CLA. A crude mixture of these isomers has in animal studies been shown to alter body composition by a reduction in body fat mass as well as an increase in lean body mass, with the trans10, cis12 isomer having the most pronounced effect. The objective of this study was to establish the molecular mechanisms by which trans10, cis12 CLA affects lipid accumulation in adipocytes. We have shown that trans10, cis12 CLA prevents lipid accumulation in human and mouse adipocytes at concentrations as low as 5 and 25 micromolar, respectively. Trans10, cis12 CLA fails to activate peroxisome proliferator-activated receptor gamma (PPARgamma), but selectively inhibits thiazolidinedione-induced PPARgamma activation in 3T3-L1 adipocytes. Treatment of mature adipocytes with trans10, cis12 CLA alone, or in combination with Darglitazone, down regulate the mRNA expression of PPARgamma, as well as its target genes fatty acid binding protein (aP2) and liver X receptor alpha (LXRalpha). Taken together, our results suggest that the trans10, cis12 CLA isomer prevents lipid accumulation in adipocytes by acting as a PPARgamma modulator.
Revised on May 12, 2003
Accepted on May 13, 2003
Trans10, cis12 conjugated linoleic acid prevents triacylglycerol accumulation in adipocytes by acting as a PPARgamma modulator
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  J. R. Miller, P. Siripurkpong, J. Hawes, A. Majdalawieh, H.-S. Ro, and R. S. McLeod The trans-10, cis-12 isomer of conjugated linoleic acid decreases adiponectin assembly by PPAR{gamma}-dependent and PPAR{gamma}-independent mechanisms J. Lipid Res., March 1, 2008; 49(3): 550 - 562. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Kennedy, S. Chung, K. LaPoint, O. Fabiyi, and M. K. McIntosh Trans-10, Cis-12 Conjugated Linoleic Acid Antagonizes Ligand-Dependent PPAR{gamma} Activity in Primary Cultures of Human Adipocytes J. Nutr., March 1, 2008; 138(3): 455 - 461. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. A. Spector and A. W. Norris Action of epoxyeicosatrienoic acids on cellular function Am J Physiol Cell Physiol, March 1, 2007; 292(3): C996 - C1012. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. S.W. Taylor, S. R.P. Williams, R. Rhys, P. James, and M. P. Frenneaux Conjugated Linoleic Acid Impairs Endothelial Function Arterioscler. Thromb. Vasc. Biol., February 1, 2006; 26(2): 307 - 312. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. L. House, J. P. Cassady, E. J. Eisen, T. E. Eling, J. B. Collins, S. F. Grissom, and J. Odle Functional genomic characterization of delipidation elicited by trans-10, cis-12-conjugated linoleic acid (t10c12-CLA) in a polygenic obese line of mice Physiol Genomics, May 11, 2005; 21(3): 351 - 361. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. A. Sauer, R. T. Dauchy, D. E. Blask, J. A. Krause, L. K. Davidson, E. M. Dauchy, K. J. Welham, and K. Coupland Conjugated Linoleic Acid Isomers and Trans Fatty Acids Inhibit Fatty Acid Transport in Hepatoma 7288CTC and Inguinal Fat Pads in Buffalo Rats J. Nutr., August 1, 2004; 134(8): 1989 - 1997. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J.-M. Gaullier, J. Halse, K. Hoye, K. Kristiansen, H. Fagertun, H. Vik, and O. Gudmundsen Conjugated linoleic acid supplementation for 1 y reduces body fat mass in healthy overweight humans Am. J. Clinical Nutrition, June 1, 2004; 79(6): 1118 - 1125. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. G Taylor and P. Zahradka Dietary conjugated linoleic acid and insulin sensitivity and resistance in rodent models Am. J. Clinical Nutrition, June 1, 2004; 79(6): 1164S - 1168S. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. M. Larsen, S. Toubro, and A. Astrup Efficacy and safety of dietary supplements containing CLA for the treatment of obesity: evidence from animal and human studies J. Lipid Res., December 1, 2003; 44(12): 2234 - 2241. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. M. Brown and M. K. McIntosh Conjugated Linoleic Acid in Humans: Regulation of Adiposity and Insulin Sensitivity J. Nutr., October 1, 2003; 133(10): 3041 - 3046. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
| All ASBMB Journals | Journal of Biological Chemistry |
| Molecular and Cellular Proteomics | ASBMB Today |
