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Papers In Press, published online ahead of print June 1, 2003
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Department of Molecular Biology and Immunology, University of North Texas Health Science Center, Fort Worth, TX 76107-2699
Corresponding Author: ldory{at}hsc.unt.edu
Macrophage apoptosis is an important factor in determining the efficiency of the immune response, atherosclerotic lesion stability and clearance of aged cells by phagocytosis. The involvement of caveolin-1 in the regulation of apoptosis has been previously suggested in fibroblasts and epithelial cells. Here we show that treatment of thioglycollate-elicited mouse peritoneal macrophages with various unrelated apoptotic agents, including simvastatin, camptothecin or glucose deprivation, is associated with a specific and large increase in caveolin-1 expression. In contrast, caveolin-2 levels remain unaffected. Induction of apoptosis, was measured by changes in cell morphology, annexin V-labeling and DNA fragmentation. We demonstrate that caveolin-1 in macrophages is present in lipid rafts and co-localizes with phosphatidylserine at the cell surface of apoptotic macrophages. Our data suggest that caveolin-1 increase is an early event, closely accompanied by phosphatidylserine externalization and independent of caspase activation and nuclear DNA fragmentation. The increase in caveolin-1 levels does not require new protein synthesis, as cycloheximide does not prevent the apoptosis- mediated increase in caveolin-1 levels. We propose that increased levels of caveolin-1 characterize the apoptotic phenotype of macrophages. Caveolin-1 may be involved in the efficient externalization of phosphatidylserine at the surface of the apoptotic cells.
Revised on May 19, 2003
Accepted on May 28, 2003
Cellular apoptosis is associated with increased caveolin-1 expression in macrophages
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