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Papers In Press, published online ahead of print August 16, 2003
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Department of Oxford Centre for Diabetes, Endocrinology and Metabolism, University of Oxford, Oxford, Oxford OX3 7LJ
Corresponding Author: fredrik.karpe{at}oxlip.ox.ac.uk
Circulating triacylglycerol arises mainly from dietary fat. However, little is known about the entry of dietary fat into the major triacylglycerol (TG) pool, very low density lipoprotein (VLDL) TG. We used a novel method to study the specific incorporation of dietary fatty acids into postprandial VLDL-TG in humans. Eight healthy volunteers (age 25.4 ± 2.2 years, BMI 22.1 ± 2.3 kg/m2) were fed a mixed meal containing 30 g of fish oil and 600 mg [1-13C]palmitic acid. Chylomicrons and VLDL were separated using immunoaffinity against apoB-100. Fatty acid composition of lipoproteins was analysed by gas chromatography / mass spectrometry. [1-13C]Palmitic acid started to appear in VLDL-TG 3 h after meal intake and a similar delay was observed for eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA). Around 20% of dietary fatty acids entered the VLDL-TG pool 6 h after meal intake. DHA was clearly over-incorporated into this pool compared with [1-13C]palmitic acid and EPA. This seemed to depend on a marked elevation of this fatty acid in the non-esterified fatty acid pool. In summary, the contribution of dietary fatty acids to early postprandial VLDL-TG is substantial. The role of DHA in VLDL-TG production will require further investigation.
Revised on July 17, 2003
Accepted on August 4, 2003
Selective partitioning of dietary fatty acids into the VLDL-TG pool in the early postprandial period
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