J. Lipid Res. Did you know there is a large type edition? Click here.
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

A more recent version of this article appeared on December 1, 2003

Papers In Press, published online ahead of print August 16, 2003
J. Lipid Res., doi:10.1194/jlr.M300192-JLR200
This Article
Right arrow Full Text (Accepted Manuscript)
Right arrow All Versions of this Article:
M300192-JLR200v1
44/12/2320    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Petersen, R. K.
Right arrow Articles by Madsen, L.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Petersen, R. K.
Right arrow Articles by Madsen, L.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Submitted on May 9, 2003
Revised on August 13, 2003
Accepted on August 14, 2003

Arachidonic acid-dependent inhibition of adipocyte differentiation requires PKA activity and is associated with sustained expression of cyclooxygenases

Rasmus K. Petersen, Claus Jørgensen, Arild C. Rustan, Livar Frøyland, Karin Muller-Decker, Gerhard Furstenberger, Rolf K. Berge, Karsten Kristiansen, and Lise Madsen

Department of Biochemistry and Molecular Biology, University of Southern Denmark, Odense M DK-5230

Corresponding Author: lise.madsen{at}bmb.sdu.dk

Arachidonic acid has been shown to inhibit adipocyte differentiation of 3T3-L1 cells via a prostaglandin synthesis-dependent pathway. Accordingly, inclusion of non-selective cyclooxygenase (COX)-inhibitors was shown to rescue adipocyte differentiation in the presence of arachidonic acid. Here we show that arachidonic acid-mediated inhibition of adipocyte differentiation requires the presence of a cAMP-elevating agent in the adipogenic cocktail during the first two days of the differentiation process. Furthermore, suppression of the activity of extracellular signal-regulated kinase 1/2 (ERK1/2) or protein kinase A (PKA) by the selective inhibitors U0126 and H-89, respectively, restored the ability of arachidonic acid-treated cells to undergo differentiation. 3T3-L1 preadipocytes express both COX-isoforms. We show that expression of COX-2 is transiently induced during the first hours of the differentiation program and then declines to become almost undetectable in fully differentiated adipocytes. COX-1 expression declines gradually as differentiation progresses, but expression is still detectable in mature adipocytes. Treatment with arachidonic acid led to a sustained expression of COX-1 and COX-2. Omission of a cAMP-elevating agent or addition of U0126 or H-89 prevents sustained expression of COX-2. Surprisingly, selective inhibition of either COX-1 or COX-2 rescued adipocyte differentiation in the presence of arachidonic acid as effectively as the non-selective COX-inhibitor indomethacin. When cells were induced to differentiate in the presence of arachidonic acid de novo fatty acid synthesis, diacylglycerol acyltransferase (DGAT) activity and triacylglycerol accumulation were inhibited. Indomethacin restored DGAT activity and triacylglycerol accumulation without restoring de novo fatty acid synthesis, and under these conditions enhanced incorporation of arachidonic acid into cellular triacylglycerols was observed.


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 Mol. Cell. Biol.Home page
R. K. Petersen, L. Madsen, L. M. Pedersen, P. Hallenborg, H. Hagland, K. Viste, S. O. Doskeland, and K. Kristiansen
Cyclic AMP (cAMP)-Mediated Stimulation of Adipocyte Differentiation Requires the Synergistic Action of Epac- and cAMP-Dependent Protein Kinase-Dependent Processes
Mol. Cell. Biol., June 1, 2008; 28(11): 3804 - 3816.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
L. Madsen, L. M. Pedersen, B. Liaset, T. Ma, R. K. Petersen, S. van den Berg, J. Pan, K. Muller-Decker, E. D. Dulsner, R. Kleemann, et al.
cAMP-dependent Signaling Regulates the Adipogenic Effect of n-6 Polyunsaturated Fatty Acids
J. Biol. Chem., March 14, 2008; 283(11): 7196 - 7205.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
O. Aprikian, D. Reynaud, C. Pace-Asciak, P. Leone, F. Blancher, I. Monnard, C. Darimont, and K. Mace
Neonatal dietary supplementation of arachidonic acid increases prostaglandin levels in adipose tissue but does not promote fat mass development in guinea pigs
Am J Physiol Regulatory Integrative Comp Physiol, November 1, 2007; 293(5): R2006 - R2012.
[Abstract] [Full Text] [PDF]

Home page
 Arterioscler. Thromb. Vasc. Bio.Home page
J. Tsai, W. Qiu, R. Kohen-Avramoglu, and K. Adeli
MEK-ERK Inhibition Corrects the Defect in VLDL Assembly in HepG2 Cells: Potential Role of ERK in VLDL-ApoB100 Particle Assembly
Arterioscler. Thromb. Vasc. Biol., January 1, 2007; 27(1): 211 - 218.
[Abstract] [Full Text] [PDF]

Home page
 J. Lipid Res.Home page
F. Haugen, N. Zahid, K. T. Dalen, K. Hollung, H. I. Nebb, and C. A. Drevon
Resistin expression in 3T3-L1 adipocytes is reduced by arachidonic acid
J. Lipid Res., January 1, 2005; 46(1): 143 - 153.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
 All ASBMB Journals   Journal of Biological Chemistry 
 Molecular and Cellular Proteomics   ASBMB Today 
Copyright © 2003 by the American Society for Biochemistry and Molecular Biology.