J. Lipid Res.
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A more recent version of this article appeared on November 1, 2003

Papers In Press, published online ahead of print August 16, 2003
J. Lipid Res., doi:10.1194/jlr.M300237-JLR200
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Submitted on June 4, 2003
Revised on July 25, 2003
Accepted on August 4, 2003

Differential effects of coconut oil- and fish oil-enriched diets on the tricarboxylate carrier in rat-liver mitochondria

Anna Maria Giudetti, Simona Sabetta, Roberta di Summa, Monica Leo, Fabrizio Damiano, Luisa Siculella, and Gabriele Vincenzo Gnoni

Scienze e Tecnologie Biologiche ed Ambientali, Università di Lecce, Lecce, Lecce 73100

Corresponding Author: gabriele.gnoni{at}unile.it

The mitochondrial tricarboxylate carrier plays an important role in lipogenesis being responsible for the efflux from the mitochondria to the cytosol of acetyl-CoA, the primer for fatty acid synthesis. In this study we investigated the effects of two high fat diets with different fatty acid composition on the hepatic tricarboxylate carrier activity. Rats were fed for 3 weeks on a basal diet supplemented with 15% of either coconut oil, abundant in medium-chain saturated fatty acids or fish oil, rich in n-3 PUFA. Mitochondrial fatty acid composition was differently influenced by the dietary treatments, while no appreciable change in phospholipid composition and cholesterol level was observed. Compared to coconut oil, the tricarboxylate carrier activity was markedly decreased in liver mitochondria from fish oil-fed rats; kinetic analysis of the carrier revealed a decrease of the Vmax with no change of the Km. No difference in the Arrhenius plot between the two groups was observed. Interestingly, the carrier protein level and the corresponding mRNA abundance decreased following fish oil treatment. These data indicate that fish oil administration markedly decreased the tricarboxylate carrier activity as compared to coconut oil. This effect is most likely due to a reduced gene expression of the carrier protein.


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