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Papers In Press, published online ahead of print January 16, 2004
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Biochemistry, Cell Biology and Metabolism, Nagoya City University Graduate School of Medical Sciences, Nagoya, Aichi 467-8601
Corresponding Author: syokoyam{at}med.nagoya-cu.ac.jp
Helical apolipoproteins of high density lipoprotein (HDL) remove phospholipid and cholesterol from cells and generate HDL particles being mediated by ATP binding cassette transporter A1 (ABCA1). In murine macrophage cell line RAW264 cells, cAMP induced e xpression of ABCA1, release of cellular phospholipid and cholesterol by apolipoprotein (apo) A-I, and reversible binding of apoA-I to the cell. The apoA-I-dependent lipid release was directly proportional to the cAMP-induced binding of apoA-I, and was in hibited by 70 % by a monoclonal antibody selective to lipid-free apoA-I, 725-1E2. In contrast, apparent cellular cholesterol release to HDL was substantial even without ABCA1 induction, and it was increased only by 27 % after the cAMP treatment. The ant ibody inhibited this increment by 70 %. Lipid-free apoA-II liberated apoA-I from HDL by displacement and thereby markedly expanded the cAMP-induced part of the cholesterol release to the HDL-containing medium, and the antibody inhibited this part also by 70 %. Binding experiments of the double-labeled reconstituted HDL showed that cAMP induced reversible binding of apoA-I but not the association of cholesteryl ester with the cells. The effect of the antibody on the cellular cholesterol release to the r econstitued HDL was similar to that on the HDL-mediated relase. The data implicated that the ABCA1-dependent cholesterol release to HDL is mediated by apoA-I dissociated from HDL.m
Revised on January 12, 2004
Accepted on January 15, 2004
Potential involvement of dissociated apolipoprotein A-I in the ABCA1-dependent cellular lipid release by high density lipoprotein
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