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J. Lipid Res.
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A more recent version of this article appeared on February 1, 2004

Papers In Press, published online ahead of print November 1, 2003
J. Lipid Res., doi:10.1194/jlr.M300353-JLR200
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Submitted on August 18, 2003
Revised on October 22, 2003
Accepted on October 23, 2003

Molecular characterization of rabbit scavenger receptor class B type I and II: Portal to central vein gradient of expression in the liver

Andreas Ritsch, Ivan Tancevski, Wilfried Schgoer, Christa Pfeifhofer, Roland Gander, Philipp Eller, Bernhard Foeger, Ursula Stanzl, and Josef R. Patsch

Department of Medicine, University of Innsbruck, Innsbruck, Innsbruck A-6020

Corresponding Author: andreas.ritsch{at}uibk.ac.at

To further elucidate the role of scavenger receptor class B type I (SR-BI) in reverse cholesterol transport and in atherogenesis, we performed studies in the rabbit, an animal model displaying a lipoprotein profile similar to that of humans, expressing cholesteryl ester transfer protein in plasma and having been demonstrated to be susceptible to atherosclerosis. In this report we describe, for the first time, isolation and characterization of rabbit cDNA fragments encoding SR-BI and SR-BII, respectively. Development of an isoform specific Taqman Real Time PCR system and generation of isoform specific polyclonal antibodies allowed us to measure SR-BI/II expression in various rabbit organs on mRNA and protein levels, respectively. We found highest expression of SR-BI in adrenal gland, liver and proximal intestine, and, to a less extent, expression in appendix and spleen. Immunohistochemical staining of frozen sections showed SR-BI expression in the cortex but not the medulla of adrenals. An increasing portal to central vein gradient of expression was found within the hepatic lobule. Identification and characterization of SR-BI expression in the rabbit affords – as shown in this report – a powerful tool to elucidate the role of SR-BI in cholesterol homeostasis and atherogenesis in humans.


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