J. Lipid Res.
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

A more recent version of this article appeared on February 1, 2004

Papers In Press, published online ahead of print November 16, 2003
J. Lipid Res., doi:10.1194/jlr.M300369-JLR200
This Article
Right arrow Full Text (Accepted Manuscript)
Right arrow All Versions of this Article:
M300369-JLR200v1
45/2/295    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Mano, N.
Right arrow Articles by Goto, J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Mano, N.
Right arrow Articles by Goto, J.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Submitted on September 2, 2003
Revised on October 30, 2003
Accepted on November 3, 2003

Presence of protein-bound unconjugated bile acids in the cytoplasmic fraction of rat brain

Nariyasu Mano, Takaaki Goto, Masashi Uchida, Koji Nishimura, Masayuki Ando, Norihiro Kobayashi, and Junichi Goto

Graduate School of Pharmaceutical Sciences, Tohoku University, Sendai 980-8578

Corresponding Author: n-mano{at}mail.pharm.tohoku.ac.jp

Using liquid chromatography/electrospray ionization mass spectrometry, we have found three unconjugated bile acids cholic, chenodeoxycholic, and deoxycholic acids in the rat brain cytoplasmic fraction. Chenodeoxycholic acid was detected only upon extraction with high concentrations of guanidine, indicating that it is bound non-covalently to protein in the brain. The most abundant of the three, it was present at a concentration of 1.6 nmol/g wet weight (ca. 15 mg protein) of brain, corresponding to almost 30 times its serum concentration. Cholic and deoxycholic acids were present at one-thirtieth the concentration of chenodeoxycholic acid. Bile acids conjugated with amino acids, sulfuric acid, and glucuronic acid were not detected. The present data clearly demonstrate that unconjugated chenodeoxycholic acid, and, to a lesser extent, cholic and deoxycholic acids, exist in the rat brain.


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 J. Lipid Res.Home page
K. Karu, M. Hornshaw, G. Woffendin, K. Bodin, M. Hamberg, G. Alvelius, J. Sjovall, J. Turton, Y. Wang, and W. J. Griffiths
Liquid chromatography-mass spectrometry utilizing multi-stage fragmentation for the identification of oxysterols
J. Lipid Res., April 1, 2007; 48(4): 976 - 987.
[Abstract] [Full Text] [PDF]

Home page
 J. Lipid Res.Home page
A. K. Bhattacharyya, D. S. Lin, and W. E. Connor
Cholestanol metabolism in patients with cerebrotendinous xanthomatosis: absorption, turnover, and tissue deposition
J. Lipid Res., January 1, 2007; 48(1): 185 - 192.
[Abstract] [Full Text] [PDF]

Home page
 J. Lipid Res.Home page
P. Liere, A. Pianos, B. Eychenne, A. Cambourg, S. Liu, W. Griffiths, M. Schumacher, J. Sjovall, and E.-E. Baulieu
Novel lipoidal derivatives of pregnenolone and dehydroepiandrosterone and absence of their sulfated counterparts in rodent brain
J. Lipid Res., December 1, 2004; 45(12): 2287 - 2302.
[Abstract] [Full Text] [PDF]

Home page
 J. Lipid Res.Home page
N. Mano, Y. Sato, M. Nagata, T. Goto, and J. Goto
Bioconversion of 3{beta}-hydroxy-5-cholenoic acid into chenodeoxycholic acid by rat brain enzyme systems
J. Lipid Res., September 1, 2004; 45(9): 1741 - 1748.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
 All ASBMB Journals   Journal of Biological Chemistry 
 Molecular and Cellular Proteomics   ASBMB Today 
Copyright © 2003 by the American Society for Biochemistry and Molecular Biology.