J. Lipid Res.
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

A more recent version of this article appeared on May 1, 2004

Papers In Press, published online ahead of print February 16, 2004
J. Lipid Res., doi:10.1194/jlr.M300484-JLR200
This Article
Right arrow Full Text (Accepted Manuscript)
Right arrow All Versions of this Article:
M300484-JLR200v1
45/5/923    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by de Jager, M. W.
Right arrow Articles by Bouwstra, J. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by de Jager, M. W.
Right arrow Articles by Bouwstra, J. A.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Submitted on November 24, 2003
Revised on February 9, 2004
Accepted on February 11, 2004

Novel lipid mixtures based on synthetic ceramides reproduce the unique stratum corneum lipid organization

Miranda W. de Jager, Gert S. Gooris, Igor P. Dolbnya, Wim Bras, Maria Ponec, and Joke A. Bouwstra

Drug Delivery Technology, LACDR, Leiden NL-2300 RA

Corresponding Author: bouwstra{at}chem.leidenuniv.nl

Lipid lamellae present in the outermost layer of the skin protect the body from uncontrolled water loss. In human stratum corneum (SC) two crystalline lamellar phases are present, which contain mostly cholesterol, free fatty acids and 9 types of free ceramides. Previous studies have demonstrated that the SC lipid organization can be mimicked with model mixtures based on isolated SC lipids. However, those studies are hampered by low availability and high inter-individual variability of the native tissue. In order to elucidate the role of each lipid class in the formation of a competent skin barrier, the use of synthetic lipids would offer an alternative. The small and wide angle X-ray diffraction results of the present study show for the first time that synthetic lipid mixtures, containing only three synthetic ceramides, reflect to a high extent the SC lipid organization. Both an appropriately chosen preparation method and lipid composition promote the formation of two characteristic lamellar phases with repeat distances similar to those found in native SC. From all synthetic lipid mixtures examined, equimolar cholesterol:ceramides:free fatty acids mixtures equilibrated at 80°C resemble to the highest extent the lamellar and lateral SC lipid organization, both at room and elevated temperatures.


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 Biophys. JHome page
G. S. Gooris and J. A. Bouwstra
Infrared Spectroscopic Study of Stratum Corneum Model Membranes Prepared from Human Ceramides, Cholesterol, and Fatty Acids
Biophys. J., April 15, 2007; 92(8): 2785 - 2795.
[Abstract] [Full Text] [PDF]

Home page
 Biophys. JHome page
M. Arseneault and M. Lafleur
Cholesterol Sulfate and Ca2+ Modulate the Mixing Properties of Lipids in Stratum Corneum Model Mixtures
Biophys. J., January 1, 2007; 92(1): 99 - 114.
[Abstract] [Full Text] [PDF]

Home page
 J. Lipid Res.Home page
M. W. de Jager, G. S. Gooris, M. Ponec, and J. A. Bouwstra
Lipid mixtures prepared with well-defined synthetic ceramides closely mimic the unique stratum corneum lipid phase behavior
J. Lipid Res., December 1, 2005; 46(12): 2649 - 2656.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
 All ASBMB Journals   Journal of Biological Chemistry 
 Molecular and Cellular Proteomics   ASBMB Today 
Copyright © 2004 by the American Society for Biochemistry and Molecular Biology.