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A more recent version of this article appeared on August 1, 2004

Papers In Press, published online ahead of print June 1, 2004
J. Lipid Res., doi:10.1194/jlr.M300513-JLR200
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Submitted on December 17, 2003
Revised on May 6, 2004
Accepted on May 21, 2004

Lipid peroxidation induced by docosahexaenoic acid-enrichment modifies paracellular permeability in Caco-2 cells: protective role of taurine

Sònia Roig-Pérez, Francesc Guardiola, Miquel Moretó, and Ruth Ferrer

Departament de Fisiologia, Facultat de Farmàcia, Universitat de Barcelona, Barcelona E-08028

Corresponding Author: rutferrer{at}ub.edu

Dietary enrichment with docosahexaenoic acid (DHA) has numerous beneficial effects on health. However, the intake of high doses of polyunsaturated fatty acids can promote lipid peroxidation and the subsequent propagation of oxygen radicals. The purpose of this study was to evaluate the effect of DHA on lipid peroxidation and tight junction structure and permeability in Caco-2 cell cultures. Moreover, the effects of taurine, a functional ingredient with antioxidant properties, was also tested. Differentiated Caco-2 cell monolayers were maintained in DHA-supplemented conditions with or without added taurine. Incubation with 100 µM DHA increased lipid peroxidation and paracellular permeability, in parallel with a redistribution of tight junction proteins, occludin and ZO-1. Taurine partially prevented all these effects. The participation of reactive oxygen and nitrogen species in increased paracellular permeability was also examined using various agents that modify the formation of superoxide radical, hydrogen peroxide, nitric oxide and peroxynitrite. We conclude that hydrogen peroxide and peroxynitrite may be involved in the DHA-induced increase in paracellular permeability and that the protective role of taurine may be in part related to its capacity to counteract the effects of hydrogen peroxide.


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P. Kaur, K. Schulz, M. Aschner, and T. Syversen
Role of Docosahexaenoic Acid in Modulating Methylmercury-Induced Neurotoxicity
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[Abstract] [Full Text] [PDF]


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