| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
|
|
|
|||||||
|
|||||||||
Papers In Press, published online ahead of print March 16, 2004
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
University of British Columbia, Vancouver, British Columbia V5Z 4H4
Corresponding Author: cheryl{at}cmmt.ubc.ca
The LXR/RXR regulated gene ABCA1 effluxes cellular cholesterol and phospholipid to apolipoprotein A1 (apoA1), which is the rate-limiting step in high-density lipoprotein synthesis. The RXR pathway plays a critical role in testicular lipid trafficking, and RXRb-deficient males are sterile and accumulate lipids in Sertoli cells. Here we demonstrate that ABCA1 mRNA and protein are abundant in Sertoli cells, whereas germ cells express little ABCA1. LXR/RXR agonists stimulate ABCA1 expression in cultured Sertoli MSC1 and Leydig TM3 cell lines. However, Sertoli TM4 cells lack ABCA1, and TM4 cells or primary Sertoli cells cultured from ABCA1-/- mice both fail to efflux cholesterol to apoA1. Expression of exogenous ABCA1 restores apoA1-dependent cholesterol efflux in Sertoli TM4 cells. In vivo, ABCA1-deficient mice exhibit lipid accumulation in Sertoli cells and depletion of normal lipid droplets from Leydig cells by 2 months of age. By 6 months of age, intratesticular testosterone levels and sperm counts are significantly reduced in ABCA1-/- mice compared to wild-type controls. Finally, a 21% decrease (p=0.01) in fertility was observed between ABCA1-/- males compared to wild-type controls across their reproductive lifespans. These results show that ABCA1 plays an important role in lipid transport in Sertoli cells and influences male fertility
Revised on March 1, 2004
Accepted on March 3, 2004
The ATP-binding cassette transporter ABCA1 mediates lipid efflux from Sertoli cells and influences male fertility
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  N. Vernet, C. Dennefeld, M. Klopfenstein, A. Ruiz, D. Bok, N. B Ghyselinck, and M. Mark Retinoid X receptor beta (RXRB) expression in Sertoli cells controls cholesterol homeostasis and spermiation Reproduction, November 1, 2008; 136(5): 619 - 626. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. Peterlin, B. Zorn, M. Volk, and T. Kunej Association between the apolipoprotein B signal peptide gene insertion/deletion polymorphism and male infertility Mol. Hum. Reprod., December 1, 2006; 12(12): 777 - 779. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. D. Akpovi, S. R. Yoon, M. L. Vitale, and R-M. Pelletier The predominance of one of the SR-BI isoforms is associated with increased esterified cholesterol levels not apoptosis in mink testis J. Lipid Res., October 1, 2006; 47(10): 2233 - 2247. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Wang, H. Wang, W. Xiong, Y. Chen, Q. Ma, J. Ma, Y. Ge, and D. Han Evaluation on the phagocytosis of apoptotic spermatogenic cells by Sertoli cells in vitro through detecting lipid droplet formation by Oil Red O staining. Reproduction, September 1, 2006; 132(3): 485 - 492. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Huyghe, H. Schmalbruch, K. De Gendt, G. Verhoeven, F. Guillou, P. P. Van Veldhoven, and M. Baes Peroxisomal Multifunctional Protein 2 Is Essential for Lipid Homeostasis in Sertoli Cells and Male Fertility in Mice Endocrinology, May 1, 2006; 147(5): 2228 - 2236. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. R. Bates, J.-Q. Tao, H. L. Collins, O. L. Francone, and G. H. Rothblat Pulmonary abnormalities due to ABCA1 deficiency in mice Am J Physiol Lung Cell Mol Physiol, December 1, 2005; 289(6): L980 - L989. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. F. Oram and J. W. Heinecke ATP-Binding Cassette Transporter A1: A Cell Cholesterol Exporter That Protects Against Cardiovascular Disease Physiol Rev, October 1, 2005; 85(4): 1343 - 1372. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. Papadopoulos Cholesterol Homeostasis and Infertility: The Liver X Receptor Connection Endocrinology, June 1, 2005; 146(6): 2517 - 2518. [Full Text] [PDF]
|
|
|
|
|
|
K. M. Robertson, G. U. Schuster, K. R. Steffensen, O. Hovatta, S. Meaney, K. Hultenby, L. C. Johansson, K. Svechnikov, O. Soder, and J.-A. Gustafsson The Liver X Receptor-{beta} Is Essential for Maintaining Cholesterol Homeostasis in the Testis Endocrinology, June 1, 2005; 146(6): 2519 - 2530. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Wu, Y. Zhang, N. Wang, L. Davis, G. Yang, X. Wang, Y. Zhu, M. D. Breyer, and Y. Guan Liver X receptor-{alpha} mediates cholesterol efflux in glomerular mesangial cells Am J Physiol Renal Physiol, November 1, 2004; 287(5): F886 - F895. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
| All ASBMB Journals | Journal of Biological Chemistry |
| Molecular and Cellular Proteomics | ASBMB Today |
