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A more recent version of this article appeared on May 1, 2004

Papers In Press, published online ahead of print February 1, 2004
J. Lipid Res., doi:10.1194/jlr.M400008-JLR200
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Submitted on January 13, 2004
Revised on January 29, 2004
Accepted on January 29, 2004

Plasma phosphatidylcholine hydroperoxide as a new marker of oxidative stress in alcoholic patients

Junko Adachi, Sachio Matsushita, Naoki Yoshioka, Rika Funae, Tetsuo Fujita, Susumu Higuchi, and Yasuhiro Ueno

Department of Legal Medicine, Kobe University Graduate School of Medicine, Kobe 650-0017

Corresponding Author: adachi{at}med.kobe-u.ac.jp

Quantitative analysis of plasma phosphatidylcholine hydroperoxide (PCOOH) is an important step to evaluate the biochemical processes leading to oxidative injury. However, secondary products of lipid peroxidation are now used as indices. One hundred and nine alcoholic patients, aged 22-81 years (mean±SE, 52.0±1.3 years), and 21 healthy volunteers, aged 41-79 years (51.2±2.2 years) participated in this study. Plasma PCOOH was measured by HPLC with chemiluminescent detection. Plasma PCOOH concentration was significantly higher in alcoholic patients (46.1±4.1 pmol/ml) than controls (15.6±1.8 pmol/ml). It was significantly higher in patients with blood alcohol (88.0±10.5 pmol/ml) than those with non-alcohol (32.6±3.1 pmol/ml). The patients with high levels of aspartate aminotransferase (AST), alanine aminotransferase (ALT), gamma-glutamyl transpeptidase (gamma-GTP), and triglyceride (TG) showed significantly higher PCOOH concentration than normal levels of them. The PCOOH level was positively correlated with levels of gamma-GTP , HDL, blood alcohol concentration, and TG. Plasma PCOOH levels in 29 alcoholic patients after a six-week abstinence were decreased significantly (22.8±11.1 pmol/ml), which was associated with improvement of liver function test. This is the first measurement of plasma PCOOH in alcoholic patients. These results suggest the involvement of lipid peroxidation in alcohol-induced liver damage. These findings confirm that the PCOOH plasma concentration is a new marker of alcohol consumption as well as oxidative stress in alcoholic patients.


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