J. Lipid Res.
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A more recent version of this article appeared on August 1, 2004

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J. Lipid Res., doi:10.1194/jlr.M400036-JLR200
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Submitted on January 28, 2004
Revised on April 23, 2004
Accepted on May 25, 2004

Participation of macrophages in atherosclerotic lesion morphology in LDLr-/- mice

Natalie K. Schiller, Audrey S. Black, Gary P. Bradshaw, David J. Bonnet, and Linda K. Curtiss

Immunology, IMM-17, The Scripps Research Institute, La Jolla, CA 92037

Corresponding Author: lcurtiss{at}scripps.edu

Lyst beige mice (beige) crossed with LDL receptor-deficient mice (LDLr-/-) had a distinct atherosclerotic lesion morphology that was not observed in LDLr-/- mice. This morphology is often associated with a stable as opposed to an unstable plaque phenotype. Because of the predominance of macrophages in lesions, we hypothesized that macrophage expression of the beige mutation accounted for this distinct morphology. Both in vitro and in vivo functions of beige,LDLr-/- macrophages were studied. For the in vitro analyses, cultured bone marrow-derived macrophages from LDLr-/- and beige,LDLr-/- mice were compared. Macrophages also were compared for their ability to accumulate cholesterol, efflux cholesterol, migrate in response to chemotactic stimuli through matrigel-coated membranes and express matrix metalloproteinase 9 (MMP9). No differences in cholesterol metabolism were identified. Beige,LDLr-/- macrophage invasion in vitro appeared to be less than LDLr-/- macrophage, but did not achieve significance. Nevertheless, TNFa-induced MMP9 expression, secretion, and enzymatic activity of beige,LDLr-/- macrophages were all significantly decreased compared to LDLr-/- macrophages (p<0.05). For in vivo analyses of macrophage function, bone marrow transplantation (BMT) studies were performed. LDLr-/- mice and beige,LDLr-/- mice were irradiated and reconstituted with wild type or beige bone marrow from mice expressing green fluorescent protein (GFP). Identification of GFP cells provided for direct identification of donor-derived cells within lesions. Expression of the beige mutation in the BMT recipients, but not the BMT donors, altered the location of bone marrow derived cells and increased the collagen content of the lesions. These results suggested that impaired macrophage function by itself did not account for the stable lesion morphology of beige,LDLr-/- double-mutant mice.


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