J. Lipid Res.
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

A more recent version of this article appeared on September 1, 2004 Originally published In Press as doi:10.1194/jlr.M400069-JLR200 on June 8, 2004

Papers In Press, published online ahead of print June 1, 2004
J. Lipid Res., doi:10.1194/jlr.M400069-JLR200
This Article
Right arrow Full Text (Accepted Manuscript)
Right arrow All Versions of this Article:
M400069-JLR200v1
M400069-JLR200v2
45/9/1614    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Yu, K. C-W
Right arrow Articles by Choi, S. Y.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Yu, K. C-W
Right arrow Articles by Choi, S. Y.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Submitted on February 18, 2004
Revised on May 11, 2004
Accepted on May 28, 2004

Endothelial lipase is synthesized by hepatic and aorta endothelial cells and its expression is altered in apoE-deficient mice

Kenneth C-W Yu, Christopher David, Sujata J. Kadambi, Andreas Stahl, Ken-Ichi Hirata, Tatsuro Ishida, Thomas Quertermous, Allen D. Cooper, and Sungshin Y. Choi

Cardiovascular Biology, Palo Alto Med. Foundation, Research Institute, Palo Alto,, CA 94301

Corresponding Author: chois{at}pamfri.org

Both LPL and HL are synthesized in parenchymal cells, secreted and bind to endothelial cells. To learn where endothelial lipase (EL) is synthesized in the adult animals the localization of EL in mouse and rat liver was studied by immunohistochemical analysis. Further, to test if EL could play a role in atherogenesis, expression of EL in the aorta and liver of apoE knockout mice was determined. EL, in both mouse and rat liver was colocalized with the vascular endothelial cells and not hepatocytes. In contrast, hepatic lipase (HL) was present in both hepatocytes and endothelial cells. By in situ hybridization EL mRNA was present only in endothelial cells in liver sections. EL was also present at low levels in the aorta of normal mice. We fed apoE knockout mice (EKO) and wild-type (WT) mice a variety of diets and determined EL expression in livers and aorta. EKO showed significant expression of EL in aorta. EL expression was lower in the liver of EKO than normal mice. Cholesterol feeding lowered EL in liver of both types of mice. In the aorta EL was higher in EKO than WT mice and cholesterol feeding had no effect. Together these data suggest that EL may be up regulated at the site of the atherosclerotic lesions and thus could supply lipids to the area.


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 J. Lipid Res.Home page
K. W. S. Ko, A. Paul, K. Ma, L. Li, and L. Chan
Endothelial lipase modulates HDL but has no effect on atherosclerosis development in apoE-/- and LDLR-/- mice
J. Lipid Res., December 1, 2005; 46(12): 2586 - 2594.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
Y. Kojma, K.-i. Hirata, T. Ishida, Y. Shimokawa, N. Inoue, S. Kawashima, T. Quertermous, and M. Yokoyama
Endothelial Lipase Modulates Monocyte Adhesion to the Vessel Wall: A POTENTIAL ROLE IN INFLAMMATION
J. Biol. Chem., December 24, 2004; 279(52): 54032 - 54038.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
 All ASBMB Journals   Journal of Biological Chemistry 
 Molecular and Cellular Proteomics   ASBMB Today 
Copyright © 2004 by the American Society for Biochemistry and Molecular Biology.