J. Lipid Res.
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A more recent version of this article appeared on November 1, 2004

Papers In Press, published online ahead of print August 16, 2004
J. Lipid Res., doi:10.1194/jlr.M400100-JLR200
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Submitted on March 10, 2004
Revised on August 12, 2004
Accepted on August 13, 2004

Long-term effects of sterol depletion in caenorhabditis elegans: sterol content of synchronized wild-type and mutant populations

Mark Merris, Jessica Kraeft, G. S. Tint, and John Lenard

Physiology & Biophysics, University of Medicine & Dentistry of New Jersey, Piscataway, NJ 08854

Corresponding Author: lenard{at}umdnj.edu

Three major long-term effects of sterol deprivation in C. elegans are described. 1. The life expectancy of sterol-deprived wild-type animals is decreased by over 40%. Similar decreases are found in animals carrying mutations in the daf-9, daf-12, daf-16 and clk-1 genes, suggesting that previously described aging pathways involving these genes are not involved in the life-extending effects of sterols. 2. There is a premature loss of motility, measured by response to mild touch. 3. There is a rapid post-reproductive onset of sarcopenia (muscle wasting) as measured by total body fluorescence in a myo3::GFP expressing strain. We also report that five sterols—the desmethylsterols cholesterol, 7-dehydrocholesterol, lathosterol, and the 4a-methyl sterols lophenol and 8(14) sterol—are found in significant amounts at all stages of development and aging in cholesterol-fed animals. Supplying each of these as the sole sterol confers similar protection from the long-term effects of sterol deprivation. These findings suggest that sterols are required continuously throughout the animal’s life.


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