J. Lipid Res.
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

A more recent version of this article appeared on October 1, 2004

Papers In Press, published online ahead of print August 1, 2004
J. Lipid Res., doi:10.1194/jlr.M400181-JLR200
This Article
Right arrow Full Text (Accepted Manuscript)
Right arrow Supplemental Data
Right arrow All Versions of this Article:
M400181-JLR200v1
45/10/1899    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Meyer, A.
Right arrow Articles by Heinz, E.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Meyer, A.
Right arrow Articles by Heinz, E.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Submitted on May 11, 2004
Revised on July 20, 2004
Accepted on July 21, 2004

Novel fatty acid elongases and their use for the reconstitution of docosahexaenoic acid biosynthesis

Astrid Meyer, Helene Kirsch, Frédéric Domergue, Amine Abbadi, Petra Sperling, Jörg Bauer, Petra Cirpus, Thorsten K. Zank, Hervé Moreau, Thomas J. ;Roscoe, Ulrich Zähringer, and Ernst Heinz

Biozentrum Klein Flottbek, Universität Hamburg, Hamburg, Hamburg 22609

Corresponding Author: astrid_meyer{at}gmx.net

In algae the biosynthesis of docosahexaenoic acid (22:6omega 3, DHA) proceeds via the elongation of eicosapentaenoic acid (20:5omega 3, EPA) to 22:5omega 3, which is required as substrate for the final 4-desaturation. In order to isolate the elongase specific for this step, we searched EST and genomic databases from the algae Ostreococcus tauri and Thalassiosira pseudonana, from the fish Oncorhynchus mykiss, from the frog Xenopus laevis and from the sea squirt Ciona intestinalis using as a query the elongase sequence PpPSE1 from the moss Physcomitrella patens. The open reading frames of the identified elongase candidates were expressed in yeast for functional characterization. By this we identified two types of elongases from O. tauri and T. pseudonana: one specific for the elongation of (6-)C18-PUFAs and one specific for (5-)C20-PUFAs, showing highest activity with EPA. The clones isolated from O. mykiss, X. laevis and C. intestinalis accepted both C18- and C20-PUFAs. By coexpression of the 6- and 5-elongases from T. pseudonana and O. tauri, respectively, with the 5- and 4-desaturases from two other algae, we successfully implemented DHA synthesis in stearidonic acid-fed yeast. This may be considered as encouraging first step in future efforts to implement this biosynthetic sequence into transgenic oilseed crops.


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 Proc. Natl. Acad. Sci. USAHome page
M.-P. Agbaga, R. S. Brush, M. N. A. Mandal, K. Henry, M. H. Elliott, and R. E. Anderson
Role of Stargardt-3 macular dystrophy protein (ELOVL4) in the biosynthesis of very long chain fatty acids
PNAS, September 2, 2008; 105(35): 12843 - 12848.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
M. Hoffmann, M. Wagner, A. Abbadi, M. Fulda, and I. Feussner
Metabolic Engineering of {omega}3-Very Long Chain Polyunsaturated Fatty Acid Production by an Exclusively Acyl-CoA-dependent Pathway
J. Biol. Chem., August 15, 2008; 283(33): 22352 - 22362.
[Abstract] [Full Text] [PDF]

Home page
 Eukaryot CellHome page
J. M. Fritzler, J. J. Millership, and G. Zhu
Cryptosporidium parvum Long-Chain Fatty Acid Elongase
Eukaryot. Cell, November 1, 2007; 6(11): 2018 - 2028.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
T. Tonon, R. Qing, D. Harvey, Y. Li, T. R. Larson, and I. A. Graham
Identification of a Long-Chain Polyunsaturated Fatty Acid Acyl-Coenzyme A Synthetase from the Diatom Thalassiosira pseudonana
Plant Physiology, May 1, 2005; 138(1): 402 - 408.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
A. R. Grossman
Paths toward Algal Genomics
Plant Physiology, February 1, 2005; 137(2): 410 - 427.
[Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
 All ASBMB Journals   Journal of Biological Chemistry 
 Molecular and Cellular Proteomics   ASBMB Today 
Copyright © 2004 by the American Society for Biochemistry and Molecular Biology.