J. Lipid Res.
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A more recent version of this article appeared on December 1, 2004

Papers In Press, published online ahead of print October 1, 2004
J. Lipid Res., doi:10.1194/jlr.M400197-JLR200
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Submitted on May 27, 2004
Revised on September 19, 2004
Accepted on September 19, 2004

Evidence for altered positional specificity of LCAT in vivo: Increased synthesis of saturated cholesteryl esters after dietary supplementation with docosahexaenoic acid

Papasani V. Subbaiah, Jennifer M. Sowa, and Michael H. Davidson

Department of Medicine, University of Illinois at Chicago, Chicago, IL 60612

Corresponding Author: psubbaia{at}uic.edu

The percentage of saturated cholesteryl esters (CE) synthesized by human LCAT is several times higher than expected from the sn-2 acyl composition of plasma phosphatidylcholine (PC), whereas the synthesis of 20:4 CE and 22:6 CE is much lower than expected. To explain these discrepancies, we proposed that LCAT transfers some saturated fatty acids from the sn-1 position of PC species that contain 20:4 or 22:6 at sn-2. The present studies provide in vivo evidence for this hypothesis. We determined the composition and synthesis of CE species in plasma of volunteers before and after a 6 week dietary supplementation with docosahexaenoic acid (22:6, DHA). In addition to an increase in the DHA content of all plasma lipids, there was a significant (+12%, p<0.005) increase of 16:0 CE, although there was no increase in 16:0 at sn-2 of PC. The increase of DHA in CE was much lower than its increase in sn-2 of PC. Ex vivo synthesis of CE species in plasma showed a significant (+24%, p<0.005) increase in the synthesis of 16:0 CE after DHA supplementation, which correlated positively with the increase of 22:6, but not of 16:0, at sn-2 of PC. These results show that the positional specificity of human LCAT is altered when the concentration of 16:0-22:6 PC is increased by DHA supplementation.


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D. K. Singh and P. V. Subbaiah
Modulation of the activity and arachidonic acid selectivity of group X secretory phospholipase A2 by sphingolipids
J. Lipid Res., March 1, 2007; 48(3): 683 - 692.
[Abstract] [Full Text] [PDF]


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