J. Lipid Res.
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A more recent version of this article appeared on December 1, 2004

Papers In Press, published online ahead of print September 1, 2004
J. Lipid Res., doi:10.1194/jlr.M400289-JLR200
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Submitted on July 30, 2004
Revised on August 26, 2004
Accepted on August 27, 2004

Validation of deuterium labeled fatty acids for the measurement of dietary fat oxidation during physical activity

Aarthi Raman, Stephane Blanc, Alexandra Adams, and Dale A. Schoeller

Department of Nutritional Sciences, University of Wisconsin at Madison, Madison, WI 53706

Corresponding Author: dschoell{at}nutrisci.wisc.edu

Measurement of 13C-labeled fatty acid oxidation is hindered by the need for acetate correction, measurement of VCO2 in a controlled environment and frequent collection of breath samples. The use of deuterium labeled fatty acids may over come these limitations. Herein, d31-palmitate was validated against [1-13C]-palmitate during exercise. Thirteen subjects with BMI = 22.9  3 kg/m2 and % body fat = 19.6  11% were subjected to 2 or 4 hours of exercise at 25% VO2 max. The d31-palmitate and [1-13C]-palmitate were given orally, in a liquid meal at breakfast. The d3-acetate and [1-13C]-acetate were given during another visit for acetate sequestration correction. Recovery of d31-palmitate in urinary water at 9 h post-dose was compared to [1-13C]-palmitate recovery in breath. Cumulative recovery of d31-palmitate was 10.6  3% and that of [1-13C]-palmitate was 5.6  2%. The d3-acetate and [1-13C]-acetate recoveries were 85  4% and 54  4% respectively. When [1-13C]-acetate recovery was used to correct 13C data, the average % recovery differences were 0.4  3%. Uncorrected d31-palmitate and group-acetate corrected [1-13C]-palmitate were well correlated (y = 0.96x + 0; P <0.0001) when used to measure fatty acid oxidation during exercise. Thus d31-palmitate can be used in outpatient settings as it eliminates the need for acetate correction and frequent sampling.


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A. Bergouignan, D. A. Schoeller, S. Votruba, C. Simon, and S. Blanc
The acetate recovery factor to correct tracer-derived dietary fat oxidation in humans
Am J Physiol Endocrinol Metab, April 1, 2008; 294(4): E645 - E653.
[Abstract] [Full Text] [PDF]


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