J. Lipid Res.
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A more recent version of this article appeared on April 1, 2005

Papers In Press, published online ahead of print January 16, 2005
J. Lipid Res., doi:10.1194/jlr.M400370-JLR200
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Submitted on September 27, 2004
Revised on December 27, 2004
Accepted on January 11, 2005

Expression, activity, and cellular localization of hormone-sensitive lipase in rat mammary gland during pregnancy and lactation

Antonia Martín-Hidalgo, Lydia Huerta, Nieves Álvarez, Gemma Alegría, María del Val Toledo, and Emilio Herrera

Bioquimica, Biologia Molecular y Biologia Celular, Universidad San Pablo-CEU, Boadilla del Monte, Madrid E-28668

Corresponding Author: eherrera{at}ceu.es

Hormone-sensitive lipase (HSL) expression and activity have been demonstrated in different tissues. Here we examined the presence of HSL in mammary glands of non-pregnant (virgin), pregnant (12, 20 and 21 days) and lactating (1 and 4 days postpartum) rats. Immunohistochemistry with antibody against rat HSL always revealed positive HSL-like immunoreactivity in the cytoplasm of both alveolar epithelial cells and adipocytes. In virgin rats, positive immunoreactivity for HSL was observed in mammary adipocytes, whereas light, diffuse staining was found in the epithelial cells. The positive staining for HSL remains in the two types of cells in mammary glands of pregnant and lactating rats. However, as pregnancy advances, the staining intensity and the number of positive immunoreactivity for HSL increased in the epithelial cells parallel to their proliferation, attaining the maximum during lactation. Western blotting analysis revealed an immunoreactive band of 84 kDa in the rat mammary gland, the same size as was found in rat white fat HSL. An HSL mRNA of 3.3 kb was also found in the rat mammary gland as in white adipose tissue. Both HSL protein and activity were lower in mammary glands from 20 and 21 day pregnant rats than those of virgin rats, although they returned to virgin values on days 1 and 4 of lactation. Values of mammary gland HSL activity of all the individual animals correlated negatively to plasma insulin levels. Immunoreactive HSL and HSL activity were found in lactating rats’ milk. In conclusion, the present results demonstrate the presence of HSL in both epithelial cells and adipocytes in mammary glands of pregnant and lactating rats, and the changes observed indicate an active role in mammary gland lipid metabolism.


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